Strain Fitness in Shewanella oneidensis MR-1 around SO0045

Experiment: Fumarate electron acceptor; ground control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSp5SC and tRNA-Asp-1 are separated by 34 nucleotidestRNA-Asp-1 and SO0044 are separated by 440 nucleotidesSO0044 and SO0045 are separated by 24 nucleotidesSO0045 and SO0046 overlap by 26 nucleotidesSO0046 and SO0047 are separated by 9 nucleotides SO_r003: Sp5SC - 5S ribosomal RNA (RefSeq), at 51,057 to 51,176 Sp5SC SO_t001: tRNA-Asp-1 - tRNA-Asp (RefSeq), at 51,211 to 51,287 tRNA-Asp-1 SO0044: SO0044 - conserved hypothetical protein (NCBI ptt file), at 51,728 to 51,979 SO0044 SO0045: SO0045 - Rrf2 family protein (NCBI ptt file), at 52,004 to 52,459 SO0045 SO0046: SO0046 - conserved hypothetical protein (NCBI ptt file), at 52,434 to 53,189 SO0046 SO0047: SO0047 - carboxyl-terminal protease, putative (NCBI ptt file), at 53,199 to 54,407 SO0047 Position (kb) 52 53Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 51.359 kb on + strandat 51.367 kb on - strandat 51.497 kb on - strandat 51.498 kb on - strandat 51.556 kb on + strandat 51.665 kb on - strandat 51.745 kb on - strandat 51.815 kb on + strand, within SO0044at 51.823 kb on - strand, within SO0044at 51.823 kb on - strand, within SO0044at 51.832 kb on + strand, within SO0044at 51.832 kb on + strand, within SO0044at 51.840 kb on - strand, within SO0044at 51.906 kb on + strand, within SO0044at 52.028 kb on - strandat 52.045 kb on + strandat 52.289 kb on + strand, within SO0045at 52.351 kb on - strand, within SO0045at 52.353 kb on + strand, within SO0045at 52.519 kb on - strand, within SO0046at 52.589 kb on + strand, within SO0046at 52.709 kb on + strand, within SO0046at 52.759 kb on + strand, within SO0046at 52.830 kb on + strand, within SO0046at 52.870 kb on - strand, within SO0046at 52.935 kb on + strand, within SO0046at 52.935 kb on + strand, within SO0046at 52.943 kb on - strand, within SO0046at 52.943 kb on - strand, within SO0046at 52.966 kb on + strand, within SO0046at 52.990 kb on + strand, within SO0046at 52.998 kb on - strand, within SO0046at 53.005 kb on + strand, within SO0046at 53.013 kb on - strand, within SO0046at 53.013 kb on - strand, within SO0046at 53.022 kb on + strand, within SO0046at 53.030 kb on - strand, within SO0046at 53.142 kb on - strandat 53.144 kb on + strandat 53.152 kb on - strandat 53.152 kb on - strandat 53.254 kb on - strandat 53.334 kb on - strand, within SO0047at 53.346 kb on - strand, within SO0047at 53.382 kb on + strand, within SO0047at 53.389 kb on + strand, within SO0047at 53.390 kb on - strand, within SO0047at 53.428 kb on + strand, within SO0047at 53.441 kb on + strand, within SO0047

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Per-strain Table

Position Strand Gene LocusTag Fraction Fumarate electron acceptor; ground control
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51,359 + +2.7
51,367 - +0.0
51,497 - -2.0
51,498 - +0.2
51,556 + -1.4
51,665 - +2.1
51,745 - -2.7
51,815 + SO0044 0.35 -1.9
51,823 - SO0044 0.38 +1.7
51,823 - SO0044 0.38 +0.6
51,832 + SO0044 0.41 -1.4
51,832 + SO0044 0.41 +0.4
51,840 - SO0044 0.44 +1.1
51,906 + SO0044 0.71 -1.0
52,028 - -2.9
52,045 + -1.4
52,289 + SO0045 0.62 -1.7
52,351 - SO0045 0.76 -1.8
52,353 + SO0045 0.77 -0.9
52,519 - SO0046 0.11 +0.0
52,589 + SO0046 0.21 +1.0
52,709 + SO0046 0.36 +0.6
52,759 + SO0046 0.43 +1.7
52,830 + SO0046 0.52 +1.0
52,870 - SO0046 0.58 +0.1
52,935 + SO0046 0.66 +0.9
52,935 + SO0046 0.66 +0.4
52,943 - SO0046 0.67 -0.7
52,943 - SO0046 0.67 -2.3
52,966 + SO0046 0.70 -3.3
52,990 + SO0046 0.74 -0.1
52,998 - SO0046 0.75 +2.7
53,005 + SO0046 0.76 +1.9
53,013 - SO0046 0.77 +1.2
53,013 - SO0046 0.77 -1.2
53,022 + SO0046 0.78 -2.6
53,030 - SO0046 0.79 +0.4
53,142 - -0.6
53,144 + -1.2
53,152 - +0.6
53,152 - -3.0
53,254 - +2.0
53,334 - SO0047 0.11 -2.7
53,346 - SO0047 0.12 -0.8
53,382 + SO0047 0.15 +1.6
53,389 + SO0047 0.16 +1.9
53,390 - SO0047 0.16 +1.2
53,428 + SO0047 0.19 -1.3
53,441 + SO0047 0.20 -2.1

Or see this region's nucleotide sequence