Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08775

Experiment: Fungal_growth_media_mCAFEs_v1 with Carbenicillin 0.008 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08765 and BWI76_RS08770 overlap by 17 nucleotidesBWI76_RS08770 and BWI76_RS08775 overlap by 8 nucleotidesBWI76_RS08775 and BWI76_RS08780 overlap by 59 nucleotides BWI76_RS08765: BWI76_RS08765 - 8-amino-7-oxononanoate synthase, at 1,847,450 to 1,848,607 _RS08765 BWI76_RS08770: BWI76_RS08770 - malonyl-ACP O-methyltransferase BioC, at 1,848,591 to 1,849,346 _RS08770 BWI76_RS08775: BWI76_RS08775 - dethiobiotin synthase, at 1,849,339 to 1,850,061 _RS08775 BWI76_RS08780: BWI76_RS08780 - ABC transporter, at 1,850,003 to 1,850,725 _RS08780 Position (kb) 1849 1850 1851Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1848.463 kb on + strand, within BWI76_RS08765at 1848.539 kb on - strandat 1848.691 kb on + strand, within BWI76_RS08770at 1848.740 kb on + strand, within BWI76_RS08770at 1848.968 kb on + strand, within BWI76_RS08770at 1849.437 kb on - strand, within BWI76_RS08775at 1849.551 kb on + strand, within BWI76_RS08775at 1849.559 kb on - strand, within BWI76_RS08775at 1849.794 kb on + strand, within BWI76_RS08775at 1849.817 kb on - strand, within BWI76_RS08775at 1850.085 kb on + strand, within BWI76_RS08780at 1850.272 kb on - strand, within BWI76_RS08780at 1850.583 kb on + strand, within BWI76_RS08780at 1850.997 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Carbenicillin 0.008 mg/mL
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1,848,463 + BWI76_RS08765 0.87 +1.3
1,848,539 - +0.3
1,848,691 + BWI76_RS08770 0.13 +0.2
1,848,740 + BWI76_RS08770 0.20 +1.4
1,848,968 + BWI76_RS08770 0.50 -0.3
1,849,437 - BWI76_RS08775 0.14 -2.0
1,849,551 + BWI76_RS08775 0.29 +0.0
1,849,559 - BWI76_RS08775 0.30 +0.6
1,849,794 + BWI76_RS08775 0.63 +0.6
1,849,817 - BWI76_RS08775 0.66 -0.8
1,850,085 + BWI76_RS08780 0.11 -1.1
1,850,272 - BWI76_RS08780 0.37 +3.1
1,850,583 + BWI76_RS08780 0.80 +0.7
1,850,997 - +0.1

Or see this region's nucleotide sequence