Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20630

Experiment: Fungal_growth_media_mCAFEs_v1 with Phosphomycin 0.01 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20625 and BWI76_RS20630 are separated by 74 nucleotidesBWI76_RS20630 and BWI76_RS20635 are separated by 230 nucleotides BWI76_RS20625: BWI76_RS20625 - DNA ligase (NAD(+)) LigA, at 4,314,962 to 4,316,986 _RS20625 BWI76_RS20630: BWI76_RS20630 - cell division protein ZipA, at 4,317,061 to 4,318,056 _RS20630 BWI76_RS20635: BWI76_RS20635 - sulfate transporter CysZ, at 4,318,287 to 4,319,048 _RS20635 Position (kb) 4317 4318 4319Strain fitness (log2 ratio) -1 0 1at 4317.073 kb on - strandat 4318.350 kb on - strandat 4318.443 kb on + strand, within BWI76_RS20635at 4318.539 kb on - strand, within BWI76_RS20635at 4318.624 kb on - strand, within BWI76_RS20635at 4318.864 kb on - strand, within BWI76_RS20635at 4318.897 kb on + strand, within BWI76_RS20635at 4318.970 kb on - strand, within BWI76_RS20635

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Phosphomycin 0.01 mg/mL
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4,317,073 - +0.6
4,318,350 - +1.0
4,318,443 + BWI76_RS20635 0.20 -0.7
4,318,539 - BWI76_RS20635 0.33 +0.6
4,318,624 - BWI76_RS20635 0.44 +0.7
4,318,864 - BWI76_RS20635 0.76 +0.3
4,318,897 + BWI76_RS20635 0.80 +0.5
4,318,970 - BWI76_RS20635 0.90 -0.6

Or see this region's nucleotide sequence