Experiment: Fungal_growth_media_mCAFEs_v1 with Phosphomycin 0.01 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17915 and BWI76_RS17920 are separated by 3 nucleotides BWI76_RS17920 and BWI76_RS17925 are separated by 60 nucleotides BWI76_RS17925 and BWI76_RS17930 are separated by 190 nucleotides BWI76_RS17930 and BWI76_RS17935 are separated by 41 nucleotides BWI76_RS17935 and BWI76_RS17940 overlap by 39 nucleotides
BWI76_RS17915: BWI76_RS17915 - membrane protein, at 3,725,209 to 3,725,775
_RS17915
BWI76_RS17920: BWI76_RS17920 - 23S rRNA (guanine(745)-N(1))-methyltransferase, at 3,725,779 to 3,726,573
_RS17920
BWI76_RS17925: BWI76_RS17925 - peptidoglycan synthase, at 3,726,634 to 3,728,379
_RS17925
BWI76_RS17930: BWI76_RS17930 - cold-shock protein CspC, at 3,728,570 to 3,728,779
_RS17930
BWI76_RS17935: BWI76_RS17935 - DUF2627 domain-containing protein, at 3,728,821 to 3,728,937
_RS17935
BWI76_RS17940: BWI76_RS17940 - hypothetical protein, at 3,728,899 to 3,729,093
_RS17940
Position (kb)
3726
3727
3728
3729 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3725.728 kb on - strand at 3725.743 kb on + strand at 3725.926 kb on - strand, within BWI76_RS17920 at 3725.979 kb on + strand, within BWI76_RS17920 at 3726.603 kb on + strand at 3726.611 kb on - strand at 3726.993 kb on - strand, within BWI76_RS17925 at 3727.153 kb on + strand, within BWI76_RS17925 at 3727.353 kb on + strand, within BWI76_RS17925 at 3727.368 kb on - strand, within BWI76_RS17925 at 3727.649 kb on + strand, within BWI76_RS17925 at 3727.657 kb on - strand, within BWI76_RS17925 at 3727.723 kb on + strand, within BWI76_RS17925 at 3727.943 kb on - strand, within BWI76_RS17925 at 3727.967 kb on + strand, within BWI76_RS17925 at 3728.292 kb on + strand at 3728.333 kb on - strand at 3728.606 kb on - strand, within BWI76_RS17930 at 3728.684 kb on - strand, within BWI76_RS17930 at 3728.687 kb on - strand, within BWI76_RS17930 at 3728.733 kb on + strand, within BWI76_RS17930 at 3728.741 kb on - strand, within BWI76_RS17930 at 3728.763 kb on + strand at 3728.771 kb on - strand at 3728.771 kb on - strand at 3728.771 kb on - strand at 3728.797 kb on - strand at 3728.826 kb on - strand at 3728.860 kb on + strand, within BWI76_RS17935 at 3728.868 kb on - strand, within BWI76_RS17935 at 3728.868 kb on - strand, within BWI76_RS17935 at 3728.913 kb on - strand at 3728.943 kb on + strand, within BWI76_RS17940 at 3728.943 kb on - strand, within BWI76_RS17940 at 3728.946 kb on - strand, within BWI76_RS17940 at 3728.951 kb on - strand, within BWI76_RS17940 at 3728.951 kb on - strand, within BWI76_RS17940 at 3728.954 kb on - strand, within BWI76_RS17940 at 3729.014 kb on + strand, within BWI76_RS17940 at 3729.014 kb on + strand, within BWI76_RS17940 at 3729.014 kb on + strand, within BWI76_RS17940 at 3729.014 kb on + strand, within BWI76_RS17940 at 3729.014 kb on + strand at 3729.014 kb on + strand, within BWI76_RS17940 at 3729.014 kb on + strand, within BWI76_RS17940 at 3729.022 kb on - strand, within BWI76_RS17940 at 3729.022 kb on - strand, within BWI76_RS17940 at 3729.022 kb on - strand, within BWI76_RS17940 at 3729.022 kb on - strand, within BWI76_RS17940 at 3729.022 kb on - strand, within BWI76_RS17940 at 3729.022 kb on - strand, within BWI76_RS17940 at 3729.041 kb on - strand, within BWI76_RS17940 at 3729.057 kb on + strand, within BWI76_RS17940 at 3729.156 kb on - strand at 3729.185 kb on - strand at 3729.202 kb on + strand at 3729.213 kb on + strand at 3729.235 kb on - strand at 3729.317 kb on + strand at 3729.317 kb on + strand at 3729.325 kb on - strand at 3729.352 kb on + strand at 3729.362 kb on + strand at 3729.362 kb on + strand at 3729.365 kb on + strand at 3729.370 kb on - strand at 3729.370 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Phosphomycin 0.01 mg/mL remove 3,725,728 - -2.8 3,725,743 + -0.6 3,725,926 - BWI76_RS17920 0.18 -0.1 3,725,979 + BWI76_RS17920 0.25 -0.4 3,726,603 + -0.4 3,726,611 - -0.7 3,726,993 - BWI76_RS17925 0.21 -0.2 3,727,153 + BWI76_RS17925 0.30 -1.7 3,727,353 + BWI76_RS17925 0.41 +0.1 3,727,368 - BWI76_RS17925 0.42 +0.5 3,727,649 + BWI76_RS17925 0.58 +0.6 3,727,657 - BWI76_RS17925 0.59 +0.0 3,727,723 + BWI76_RS17925 0.62 +0.1 3,727,943 - BWI76_RS17925 0.75 -0.9 3,727,967 + BWI76_RS17925 0.76 +0.7 3,728,292 + +0.9 3,728,333 - +0.1 3,728,606 - BWI76_RS17930 0.17 +0.2 3,728,684 - BWI76_RS17930 0.54 +0.6 3,728,687 - BWI76_RS17930 0.56 +1.4 3,728,733 + BWI76_RS17930 0.78 +0.3 3,728,741 - BWI76_RS17930 0.81 -0.3 3,728,763 + -1.1 3,728,771 - -0.9 3,728,771 - -0.8 3,728,771 - -0.2 3,728,797 - -1.1 3,728,826 - -0.3 3,728,860 + BWI76_RS17935 0.33 +0.9 3,728,868 - BWI76_RS17935 0.40 +0.6 3,728,868 - BWI76_RS17935 0.40 +0.6 3,728,913 - +0.6 3,728,943 + BWI76_RS17940 0.23 +0.9 3,728,943 - BWI76_RS17940 0.23 +0.5 3,728,946 - BWI76_RS17940 0.24 -0.1 3,728,951 - BWI76_RS17940 0.27 +0.1 3,728,951 - BWI76_RS17940 0.27 +0.3 3,728,954 - BWI76_RS17940 0.28 -3.0 3,729,014 + BWI76_RS17940 0.59 +0.9 3,729,014 + BWI76_RS17940 0.59 +0.3 3,729,014 + BWI76_RS17940 0.59 -1.0 3,729,014 + BWI76_RS17940 0.59 -2.4 3,729,014 + -1.7 3,729,014 + BWI76_RS17940 0.59 -0.9 3,729,014 + BWI76_RS17940 0.59 +0.0 3,729,022 - BWI76_RS17940 0.63 +0.1 3,729,022 - BWI76_RS17940 0.63 +0.8 3,729,022 - BWI76_RS17940 0.63 -1.1 3,729,022 - BWI76_RS17940 0.63 +1.0 3,729,022 - BWI76_RS17940 0.63 +0.6 3,729,022 - BWI76_RS17940 0.63 +1.1 3,729,041 - BWI76_RS17940 0.73 +0.6 3,729,057 + BWI76_RS17940 0.81 -1.9 3,729,156 - -1.1 3,729,185 - +0.3 3,729,202 + -2.1 3,729,213 + -0.0 3,729,235 - -0.1 3,729,317 + -2.1 3,729,317 + -0.3 3,729,325 - +0.6 3,729,352 + -0.1 3,729,362 + -0.9 3,729,362 + +0.6 3,729,365 + -2.7 3,729,370 - -0.3 3,729,370 - -0.7
Or see this region's nucleotide sequence