Experiment: L-Glutamine (C)(N); with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS11870 and PFLU_RS11875 are separated by 47 nucleotides PFLU_RS11875 and PFLU_RS11880 overlap by 4 nucleotides PFLU_RS11880 and PFLU_RS11885 overlap by 8 nucleotides PFLU_RS11885 and PFLU_RS11890 overlap by 4 nucleotides
PFLU_RS11870: PFLU_RS11870 - type II secretion system protein GspG, at 2,627,878 to 2,628,327
_RS11870
PFLU_RS11875: PFLU_RS11875 - general secretion pathway protein GspK, at 2,628,375 to 2,629,205
_RS11875
PFLU_RS11880: PFLU_RS11880 - general secretion pathway protein GspL, at 2,629,202 to 2,630,263
_RS11880
PFLU_RS11885: PFLU_RS11885 - type II secretion system protein M, at 2,630,256 to 2,630,768
_RS11885
PFLU_RS11890: PFLU_RS11890 - type II secretion system secretin GspD, at 2,630,765 to 2,633,077
_RS11890
Position (kb)
2629
2630
2631 Strain fitness (log2 ratio)
-2
-1
0
1 at 2628.214 kb on + strand, within PFLU_RS11870 at 2628.214 kb on + strand, within PFLU_RS11870 at 2628.215 kb on - strand, within PFLU_RS11870 at 2628.220 kb on + strand, within PFLU_RS11870 at 2628.325 kb on + strand at 2628.325 kb on + strand at 2628.325 kb on + strand at 2628.325 kb on + strand at 2628.325 kb on + strand at 2628.325 kb on + strand at 2628.326 kb on - strand at 2628.326 kb on - strand at 2628.326 kb on - strand at 2628.326 kb on - strand at 2628.775 kb on - strand, within PFLU_RS11875 at 2628.839 kb on - strand, within PFLU_RS11875 at 2628.839 kb on - strand, within PFLU_RS11875 at 2628.870 kb on + strand, within PFLU_RS11875 at 2628.870 kb on + strand, within PFLU_RS11875 at 2628.870 kb on + strand, within PFLU_RS11875 at 2628.870 kb on + strand, within PFLU_RS11875 at 2628.870 kb on + strand, within PFLU_RS11875 at 2628.871 kb on - strand, within PFLU_RS11875 at 2628.871 kb on - strand, within PFLU_RS11875 at 2628.871 kb on - strand, within PFLU_RS11875 at 2628.871 kb on - strand, within PFLU_RS11875 at 2628.893 kb on + strand, within PFLU_RS11875 at 2628.894 kb on - strand, within PFLU_RS11875 at 2628.894 kb on - strand, within PFLU_RS11875 at 2629.080 kb on - strand, within PFLU_RS11875 at 2629.080 kb on - strand, within PFLU_RS11875 at 2629.305 kb on - strand at 2629.305 kb on - strand at 2629.327 kb on + strand, within PFLU_RS11880 at 2629.327 kb on + strand, within PFLU_RS11880 at 2629.328 kb on - strand, within PFLU_RS11880 at 2629.452 kb on + strand, within PFLU_RS11880 at 2629.596 kb on - strand, within PFLU_RS11880 at 2629.596 kb on - strand, within PFLU_RS11880 at 2629.635 kb on - strand, within PFLU_RS11880 at 2629.853 kb on + strand, within PFLU_RS11880 at 2629.853 kb on + strand, within PFLU_RS11880 at 2630.048 kb on - strand, within PFLU_RS11880 at 2630.261 kb on + strand at 2630.377 kb on + strand, within PFLU_RS11885 at 2630.548 kb on - strand, within PFLU_RS11885 at 2630.952 kb on + strand at 2631.123 kb on + strand, within PFLU_RS11890 at 2631.176 kb on + strand, within PFLU_RS11890
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C)(N); with MOPS remove 2,628,214 + PFLU_RS11870 0.75 +0.4 2,628,214 + PFLU_RS11870 0.75 +0.9 2,628,215 - PFLU_RS11870 0.75 -1.3 2,628,220 + PFLU_RS11870 0.76 +0.4 2,628,325 + -1.4 2,628,325 + +0.4 2,628,325 + -0.9 2,628,325 + +0.4 2,628,325 + -1.4 2,628,325 + -0.9 2,628,326 - -1.9 2,628,326 - +1.4 2,628,326 - +1.1 2,628,326 - -2.6 2,628,775 - PFLU_RS11875 0.48 +0.7 2,628,839 - PFLU_RS11875 0.56 -0.1 2,628,839 - PFLU_RS11875 0.56 -1.3 2,628,870 + PFLU_RS11875 0.60 +1.4 2,628,870 + PFLU_RS11875 0.60 +0.1 2,628,870 + PFLU_RS11875 0.60 -0.3 2,628,870 + PFLU_RS11875 0.60 +0.5 2,628,870 + PFLU_RS11875 0.60 -1.9 2,628,871 - PFLU_RS11875 0.60 -1.8 2,628,871 - PFLU_RS11875 0.60 +0.3 2,628,871 - PFLU_RS11875 0.60 -0.5 2,628,871 - PFLU_RS11875 0.60 +0.7 2,628,893 + PFLU_RS11875 0.62 +0.4 2,628,894 - PFLU_RS11875 0.62 -0.1 2,628,894 - PFLU_RS11875 0.62 -0.3 2,629,080 - PFLU_RS11875 0.85 +0.3 2,629,080 - PFLU_RS11875 0.85 +0.8 2,629,305 - +0.8 2,629,305 - -1.2 2,629,327 + PFLU_RS11880 0.12 -0.2 2,629,327 + PFLU_RS11880 0.12 +0.2 2,629,328 - PFLU_RS11880 0.12 +1.4 2,629,452 + PFLU_RS11880 0.24 +0.4 2,629,596 - PFLU_RS11880 0.37 -0.3 2,629,596 - PFLU_RS11880 0.37 +0.4 2,629,635 - PFLU_RS11880 0.41 +1.4 2,629,853 + PFLU_RS11880 0.61 -0.5 2,629,853 + PFLU_RS11880 0.61 -0.4 2,630,048 - PFLU_RS11880 0.80 +0.9 2,630,261 + -0.8 2,630,377 + PFLU_RS11885 0.24 -1.8 2,630,548 - PFLU_RS11885 0.57 -0.6 2,630,952 + +0.4 2,631,123 + PFLU_RS11890 0.15 -1.0 2,631,176 + PFLU_RS11890 0.18 +0.9
Or see this region's nucleotide sequence