Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27815 and BWI76_RS27820 are separated by 102 nucleotides BWI76_RS27820 and BWI76_RS27825 are separated by 26 nucleotides BWI76_RS27825 and BWI76_RS27830 are separated by 313 nucleotides BWI76_RS27830 and BWI76_RS27835 are separated by 70 nucleotides
BWI76_RS27815: BWI76_RS27815 - acetolactate synthase catalytic subunit, at 5,770,640 to 5,772,328
_RS27815
BWI76_RS27820: BWI76_RS27820 - ilvB operon leader peptide IvbL, at 5,772,431 to 5,772,526
_RS27820
BWI76_RS27825: BWI76_RS27825 - hypothetical protein, at 5,772,553 to 5,772,795
_RS27825
BWI76_RS27830: BWI76_RS27830 - type I toxin-antitoxin system toxin TisB, at 5,773,109 to 5,773,198
_RS27830
BWI76_RS27835: BWI76_RS27835 - acyltransferase, at 5,773,269 to 5,773,715
_RS27835
Position (kb)
5772
5773 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5771.608 kb on - strand, within BWI76_RS27815 at 5771.655 kb on - strand, within BWI76_RS27815 at 5771.683 kb on + strand, within BWI76_RS27815 at 5771.691 kb on - strand, within BWI76_RS27815 at 5771.700 kb on + strand, within BWI76_RS27815 at 5771.746 kb on - strand, within BWI76_RS27815 at 5771.791 kb on + strand, within BWI76_RS27815 at 5771.846 kb on + strand, within BWI76_RS27815 at 5771.873 kb on + strand, within BWI76_RS27815 at 5771.881 kb on - strand, within BWI76_RS27815 at 5771.882 kb on - strand, within BWI76_RS27815 at 5772.058 kb on + strand, within BWI76_RS27815 at 5772.077 kb on + strand, within BWI76_RS27815 at 5772.092 kb on + strand, within BWI76_RS27815 at 5772.125 kb on - strand, within BWI76_RS27815 at 5772.248 kb on + strand at 5772.499 kb on + strand, within BWI76_RS27820 at 5772.501 kb on + strand, within BWI76_RS27820 at 5772.519 kb on + strand at 5772.519 kb on - strand at 5772.721 kb on + strand, within BWI76_RS27825 at 5772.729 kb on - strand, within BWI76_RS27825 at 5772.729 kb on - strand, within BWI76_RS27825 at 5772.795 kb on - strand at 5772.884 kb on - strand at 5773.054 kb on - strand at 5773.068 kb on - strand at 5773.130 kb on + strand, within BWI76_RS27830 at 5773.178 kb on + strand, within BWI76_RS27830 at 5773.178 kb on + strand, within BWI76_RS27830 at 5773.224 kb on - strand at 5773.305 kb on - strand at 5773.445 kb on - strand, within BWI76_RS27835 at 5773.488 kb on - strand, within BWI76_RS27835
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 5,771,608 - BWI76_RS27815 0.57 +1.3 5,771,655 - BWI76_RS27815 0.60 -0.1 5,771,683 + BWI76_RS27815 0.62 -0.7 5,771,691 - BWI76_RS27815 0.62 +0.9 5,771,700 + BWI76_RS27815 0.63 -0.1 5,771,746 - BWI76_RS27815 0.65 -0.9 5,771,791 + BWI76_RS27815 0.68 -0.3 5,771,846 + BWI76_RS27815 0.71 -0.1 5,771,873 + BWI76_RS27815 0.73 +0.2 5,771,881 - BWI76_RS27815 0.73 -2.7 5,771,882 - BWI76_RS27815 0.74 +0.3 5,772,058 + BWI76_RS27815 0.84 -2.2 5,772,077 + BWI76_RS27815 0.85 +2.5 5,772,092 + BWI76_RS27815 0.86 -1.9 5,772,125 - BWI76_RS27815 0.88 -0.4 5,772,248 + -0.8 5,772,499 + BWI76_RS27820 0.71 -2.4 5,772,501 + BWI76_RS27820 0.73 +0.3 5,772,519 + -0.9 5,772,519 - +0.2 5,772,721 + BWI76_RS27825 0.69 -2.3 5,772,729 - BWI76_RS27825 0.72 -0.7 5,772,729 - BWI76_RS27825 0.72 +0.2 5,772,795 - -0.3 5,772,884 - -0.3 5,773,054 - +0.4 5,773,068 - +0.9 5,773,130 + BWI76_RS27830 0.23 +0.6 5,773,178 + BWI76_RS27830 0.77 -0.5 5,773,178 + BWI76_RS27830 0.77 -0.8 5,773,224 - +0.6 5,773,305 - +0.3 5,773,445 - BWI76_RS27835 0.39 +0.2 5,773,488 - BWI76_RS27835 0.49 -1.0
Or see this region's nucleotide sequence