Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27370 and BWI76_RS27375 overlap by 23 nucleotides BWI76_RS27375 and BWI76_RS27380 are separated by 174 nucleotides BWI76_RS27380 and BWI76_RS27385 are separated by 124 nucleotides
BWI76_RS27370: BWI76_RS27370 - bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate synthase, at 5,679,856 to 5,681,067
_RS27370
BWI76_RS27375: BWI76_RS27375 - deoxyuridine 5'-triphosphate nucleotidohydrolase, at 5,681,045 to 5,681,503
_RS27375
BWI76_RS27380: BWI76_RS27380 - nucleoid occlusion factor SlmA, at 5,681,678 to 5,682,274
_RS27380
BWI76_RS27385: BWI76_RS27385 - orotate phosphoribosyltransferase, at 5,682,399 to 5,683,040
_RS27385
Position (kb)
5681
5682 Strain fitness (log2 ratio)
-2
-1
0
1 at 5681.619 kb on - strand at 5681.767 kb on + strand, within BWI76_RS27380 at 5681.770 kb on + strand, within BWI76_RS27380 at 5682.119 kb on + strand, within BWI76_RS27380 at 5682.161 kb on + strand, within BWI76_RS27380 at 5682.193 kb on - strand, within BWI76_RS27380 at 5682.350 kb on + strand at 5682.357 kb on + strand at 5682.423 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 5,681,619 - -0.9 5,681,767 + BWI76_RS27380 0.15 +0.1 5,681,770 + BWI76_RS27380 0.15 -2.2 5,682,119 + BWI76_RS27380 0.74 -1.3 5,682,161 + BWI76_RS27380 0.81 -1.0 5,682,193 - BWI76_RS27380 0.86 -0.4 5,682,350 + +0.4 5,682,357 + -0.3 5,682,423 + -0.7
Or see this region's nucleotide sequence