Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS24580

Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS24565 and BWI76_RS24570 are separated by 124 nucleotidesBWI76_RS24570 and BWI76_RS24575 are separated by 86 nucleotidesBWI76_RS24575 and BWI76_RS24580 are separated by 137 nucleotidesBWI76_RS24580 and BWI76_RS24585 are separated by 106 nucleotides BWI76_RS24565: BWI76_RS24565 - mismatch-specific DNA-glycosylase, at 5,121,438 to 5,121,944 _RS24565 BWI76_RS24570: BWI76_RS24570 - tRNA-Met, at 5,122,069 to 5,122,144 _RS24570 BWI76_RS24575: BWI76_RS24575 - hypothetical protein, at 5,122,231 to 5,122,554 _RS24575 BWI76_RS24580: BWI76_RS24580 - PTS-dependent dihydroxyacetone kinase operon transcriptional regulator DhaR, at 5,122,692 to 5,124,605 _RS24580 BWI76_RS24585: BWI76_RS24585 - glycerol dehydrogenase, at 5,124,712 to 5,125,809 _RS24585 Position (kb) 5122 5123 5124 5125Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5121.714 kb on + strand, within BWI76_RS24565at 5121.722 kb on - strand, within BWI76_RS24565at 5121.835 kb on - strandat 5121.872 kb on + strand, within BWI76_RS24565at 5121.874 kb on + strand, within BWI76_RS24565at 5121.874 kb on + strand, within BWI76_RS24565at 5121.910 kb on + strandat 5121.911 kb on + strandat 5121.918 kb on - strandat 5122.022 kb on - strandat 5122.241 kb on + strandat 5122.404 kb on - strand, within BWI76_RS24575at 5122.514 kb on - strand, within BWI76_RS24575at 5122.529 kb on - strandat 5122.565 kb on + strandat 5122.573 kb on - strandat 5122.697 kb on + strandat 5122.708 kb on + strandat 5122.708 kb on + strandat 5122.716 kb on - strandat 5123.022 kb on - strand, within BWI76_RS24580at 5123.531 kb on + strand, within BWI76_RS24580at 5123.533 kb on + strand, within BWI76_RS24580at 5123.536 kb on + strand, within BWI76_RS24580at 5123.539 kb on - strand, within BWI76_RS24580at 5123.567 kb on - strand, within BWI76_RS24580at 5123.978 kb on + strand, within BWI76_RS24580at 5124.083 kb on + strand, within BWI76_RS24580at 5124.281 kb on - strand, within BWI76_RS24580at 5124.332 kb on + strand, within BWI76_RS24580at 5124.380 kb on + strand, within BWI76_RS24580at 5124.466 kb on + strandat 5124.569 kb on - strandat 5124.609 kb on - strandat 5124.705 kb on + strandat 5124.713 kb on - strandat 5124.730 kb on - strandat 5124.749 kb on - strandat 5124.798 kb on - strandat 5124.972 kb on + strand, within BWI76_RS24585at 5125.136 kb on + strand, within BWI76_RS24585at 5125.429 kb on - strand, within BWI76_RS24585at 5125.438 kb on + strand, within BWI76_RS24585at 5125.467 kb on + strand, within BWI76_RS24585

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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5,121,714 + BWI76_RS24565 0.54 -1.2
5,121,722 - BWI76_RS24565 0.56 +0.0
5,121,835 - +0.3
5,121,872 + BWI76_RS24565 0.86 +0.6
5,121,874 + BWI76_RS24565 0.86 -0.4
5,121,874 + BWI76_RS24565 0.86 -0.1
5,121,910 + +0.2
5,121,911 + -0.6
5,121,918 - +0.6
5,122,022 - -0.0
5,122,241 + +0.6
5,122,404 - BWI76_RS24575 0.53 -0.5
5,122,514 - BWI76_RS24575 0.87 +0.3
5,122,529 - +1.3
5,122,565 + -0.0
5,122,573 - +2.3
5,122,697 + -0.4
5,122,708 + -1.3
5,122,708 + -1.0
5,122,716 - +1.3
5,123,022 - BWI76_RS24580 0.17 -0.3
5,123,531 + BWI76_RS24580 0.44 -0.3
5,123,533 + BWI76_RS24580 0.44 -0.7
5,123,536 + BWI76_RS24580 0.44 +1.3
5,123,539 - BWI76_RS24580 0.44 -1.8
5,123,567 - BWI76_RS24580 0.46 -1.3
5,123,978 + BWI76_RS24580 0.67 -0.7
5,124,083 + BWI76_RS24580 0.73 -0.7
5,124,281 - BWI76_RS24580 0.83 +1.3
5,124,332 + BWI76_RS24580 0.86 +1.3
5,124,380 + BWI76_RS24580 0.88 +0.3
5,124,466 + -2.4
5,124,569 - +1.3
5,124,609 - -2.8
5,124,705 + -0.7
5,124,713 - -1.1
5,124,730 - +0.5
5,124,749 - -1.3
5,124,798 - -1.8
5,124,972 + BWI76_RS24585 0.24 +0.2
5,125,136 + BWI76_RS24585 0.39 +1.3
5,125,429 - BWI76_RS24585 0.65 +0.3
5,125,438 + BWI76_RS24585 0.66 +1.3
5,125,467 + BWI76_RS24585 0.69 -0.3

Or see this region's nucleotide sequence