Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22425 and BWI76_RS22430 overlap by 4 nucleotides BWI76_RS22430 and BWI76_RS22435 overlap by 4 nucleotides BWI76_RS22435 and BWI76_RS22440 overlap by 7 nucleotides BWI76_RS22440 and BWI76_RS22445 are separated by 124 nucleotides
BWI76_RS22425: BWI76_RS22425 - metal ABC transporter substrate-binding protein, at 4,676,159 to 4,677,076
_RS22425
BWI76_RS22430: BWI76_RS22430 - manganese/iron transporter ATP-binding protein, at 4,677,073 to 4,677,894
_RS22430
BWI76_RS22435: BWI76_RS22435 - iron ABC transporter permease AfeC, at 4,677,891 to 4,678,739
_RS22435
BWI76_RS22440: BWI76_RS22440 - iron ABC transporter permease, at 4,678,733 to 4,679,587
_RS22440
BWI76_RS22445: BWI76_RS22445 - hypothetical protein, at 4,679,712 to 4,680,086
_RS22445
Position (kb) 4677
4678
4679 Strain fitness (log2 ratio) -1
0
1
2 at 4677.107 kb on + strand at 4677.363 kb on + strand, within BWI76_RS22430 at 4677.648 kb on + strand, within BWI76_RS22430 at 4677.811 kb on + strand, within BWI76_RS22430 at 4677.819 kb on - strand at 4678.036 kb on + strand, within BWI76_RS22435 at 4678.079 kb on - strand, within BWI76_RS22435 at 4678.174 kb on + strand, within BWI76_RS22435 at 4678.296 kb on - strand, within BWI76_RS22435 at 4678.351 kb on + strand, within BWI76_RS22435 at 4678.516 kb on - strand, within BWI76_RS22435 at 4678.561 kb on - strand, within BWI76_RS22435 at 4678.747 kb on + strand at 4678.747 kb on + strand at 4679.211 kb on + strand, within BWI76_RS22440 at 4679.272 kb on + strand, within BWI76_RS22440 at 4679.366 kb on - strand, within BWI76_RS22440 at 4679.425 kb on - strand, within BWI76_RS22440 at 4679.489 kb on + strand, within BWI76_RS22440 at 4679.642 kb on - strand at 4679.680 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 4,677,107 + +0.7 4,677,363 + BWI76_RS22430 0.35 +0.0 4,677,648 + BWI76_RS22430 0.70 -0.9 4,677,811 + BWI76_RS22430 0.90 -0.8 4,677,819 - -0.1 4,678,036 + BWI76_RS22435 0.17 +0.7 4,678,079 - BWI76_RS22435 0.22 +1.2 4,678,174 + BWI76_RS22435 0.33 +1.9 4,678,296 - BWI76_RS22435 0.48 +0.2 4,678,351 + BWI76_RS22435 0.54 -0.2 4,678,516 - BWI76_RS22435 0.74 +1.5 4,678,561 - BWI76_RS22435 0.79 -0.7 4,678,747 + -0.2 4,678,747 + +0.4 4,679,211 + BWI76_RS22440 0.56 -0.5 4,679,272 + BWI76_RS22440 0.63 -0.5 4,679,366 - BWI76_RS22440 0.74 +0.8 4,679,425 - BWI76_RS22440 0.81 -1.1 4,679,489 + BWI76_RS22440 0.88 +1.0 4,679,642 - -1.3 4,679,680 - +0.2
Or see this region's nucleotide sequence