Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21455 and BWI76_RS21460 are separated by 271 nucleotides BWI76_RS21460 and BWI76_RS21465 are separated by 261 nucleotides BWI76_RS21465 and BWI76_RS21470 overlap by 4 nucleotides BWI76_RS21470 and BWI76_RS21475 are separated by 68 nucleotides
BWI76_RS21455: BWI76_RS21455 - outer membrane protein assembly factor BamD, at 4,494,985 to 4,495,722
_RS21455
BWI76_RS21460: BWI76_RS21460 - translation inhibitor protein RaiA, at 4,495,994 to 4,496,329
_RS21460
BWI76_RS21465: BWI76_RS21465 - bifunctional chorismate mutase/prephenate dehydratase, at 4,496,591 to 4,497,751
_RS21465
BWI76_RS21470: BWI76_RS21470 - gluconolaconase, at 4,497,748 to 4,498,620
_RS21470
BWI76_RS21475: BWI76_RS21475 - bifunctional chorismate mutase/prephenate dehydrogenase, at 4,498,689 to 4,499,810
_RS21475
Position (kb) 4496
4497
4498 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4495.793 kb on - strand at 4495.796 kb on + strand at 4495.864 kb on + strand at 4495.864 kb on + strand at 4495.864 kb on + strand at 4495.871 kb on - strand at 4496.123 kb on - strand, within BWI76_RS21460 at 4496.414 kb on + strand at 4496.535 kb on - strand at 4496.535 kb on - strand at 4497.319 kb on + strand, within BWI76_RS21465 at 4497.341 kb on + strand, within BWI76_RS21465 at 4497.349 kb on - strand, within BWI76_RS21465 at 4497.354 kb on - strand, within BWI76_RS21465 at 4497.634 kb on - strand, within BWI76_RS21465 at 4497.644 kb on - strand at 4497.655 kb on + strand at 4497.709 kb on - strand at 4497.709 kb on - strand at 4497.888 kb on - strand, within BWI76_RS21470 at 4498.016 kb on + strand, within BWI76_RS21470 at 4498.440 kb on + strand, within BWI76_RS21470 at 4498.521 kb on + strand, within BWI76_RS21470 at 4498.563 kb on + strand at 4498.730 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 4,495,793 - -0.1 4,495,796 + +0.9 4,495,864 + +0.1 4,495,864 + +0.3 4,495,864 + -0.8 4,495,871 - +1.2 4,496,123 - BWI76_RS21460 0.38 -0.6 4,496,414 + +0.8 4,496,535 - -0.8 4,496,535 - -0.9 4,497,319 + BWI76_RS21465 0.63 -3.0 4,497,341 + BWI76_RS21465 0.65 -2.0 4,497,349 - BWI76_RS21465 0.65 -0.4 4,497,354 - BWI76_RS21465 0.66 -1.2 4,497,634 - BWI76_RS21465 0.90 -2.7 4,497,644 - -0.7 4,497,655 + -1.6 4,497,709 - -0.4 4,497,709 - -0.4 4,497,888 - BWI76_RS21470 0.16 -0.8 4,498,016 + BWI76_RS21470 0.31 +0.2 4,498,440 + BWI76_RS21470 0.79 -0.0 4,498,521 + BWI76_RS21470 0.89 +0.2 4,498,563 + -0.8 4,498,730 - -1.8
Or see this region's nucleotide sequence