Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20350 and BWI76_RS20355 are separated by 33 nucleotides BWI76_RS20355 and BWI76_RS20360 are separated by 199 nucleotides BWI76_RS20360 and BWI76_RS20365 overlap by 11 nucleotides
BWI76_RS20350: BWI76_RS20350 - amidophosphoribosyltransferase, at 4,259,839 to 4,261,356
_RS20350
BWI76_RS20355: BWI76_RS20355 - colicin V production protein, at 4,261,390 to 4,261,878
_RS20355
BWI76_RS20360: BWI76_RS20360 - cell division protein DedD, at 4,262,078 to 4,262,767
_RS20360
BWI76_RS20365: BWI76_RS20365 - bifunctional tetrahydrofolate synthase/dihydrofolate synthase, at 4,262,757 to 4,264,025
_RS20365
Position (kb) 4262
4263 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 4261.323 kb on + strand at 4261.470 kb on + strand, within BWI76_RS20355 at 4261.551 kb on - strand, within BWI76_RS20355 at 4261.570 kb on + strand, within BWI76_RS20355 at 4262.158 kb on + strand, within BWI76_RS20360
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 4,261,323 + -0.4 4,261,470 + BWI76_RS20355 0.16 -5.0 4,261,551 - BWI76_RS20355 0.33 -1.5 4,261,570 + BWI76_RS20355 0.37 -0.9 4,262,158 + BWI76_RS20360 0.12 -2.6
Or see this region's nucleotide sequence