Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18945 and BWI76_RS18950 overlap by 19 nucleotides BWI76_RS18950 and BWI76_RS18955 overlap by 7 nucleotides BWI76_RS18955 and BWI76_RS18960 are separated by 83 nucleotides
BWI76_RS18945: BWI76_RS18945 - 1-(5-phosphoribosyl)-5-((5- phosphoribosylamino)methylideneamino)imidazole-4- carboxamide isomerase, at 3,929,554 to 3,930,291
_RS18945
BWI76_RS18950: BWI76_RS18950 - imidazole glycerol phosphate synthase cyclase subunit, at 3,930,273 to 3,931,049
_RS18950
BWI76_RS18955: BWI76_RS18955 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase, at 3,931,043 to 3,931,642
_RS18955
BWI76_RS18960: BWI76_RS18960 - glycosyl transferase, at 3,931,726 to 3,933,045
_RS18960
Position (kb)
3931
3932 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3930.057 kb on + strand, within BWI76_RS18945 at 3930.223 kb on - strand at 3930.377 kb on + strand, within BWI76_RS18950 at 3930.426 kb on + strand, within BWI76_RS18950 at 3930.445 kb on + strand, within BWI76_RS18950 at 3930.909 kb on + strand, within BWI76_RS18950 at 3931.121 kb on + strand, within BWI76_RS18955 at 3931.498 kb on - strand, within BWI76_RS18955 at 3931.509 kb on + strand, within BWI76_RS18955 at 3931.517 kb on + strand, within BWI76_RS18955 at 3931.517 kb on + strand, within BWI76_RS18955 at 3931.640 kb on - strand at 3931.653 kb on - strand at 3931.674 kb on + strand at 3931.723 kb on - strand at 3931.762 kb on + strand at 3931.770 kb on - strand at 3931.816 kb on - strand at 3931.816 kb on - strand at 3931.910 kb on - strand, within BWI76_RS18960 at 3931.936 kb on + strand, within BWI76_RS18960 at 3932.013 kb on + strand, within BWI76_RS18960 at 3932.036 kb on + strand, within BWI76_RS18960 at 3932.045 kb on - strand, within BWI76_RS18960 at 3932.094 kb on + strand at 3932.094 kb on + strand, within BWI76_RS18960 at 3932.104 kb on - strand, within BWI76_RS18960 at 3932.112 kb on + strand, within BWI76_RS18960 at 3932.196 kb on + strand, within BWI76_RS18960 at 3932.196 kb on + strand, within BWI76_RS18960 at 3932.196 kb on + strand, within BWI76_RS18960 at 3932.196 kb on + strand, within BWI76_RS18960 at 3932.204 kb on - strand, within BWI76_RS18960 at 3932.204 kb on - strand, within BWI76_RS18960 at 3932.311 kb on - strand, within BWI76_RS18960 at 3932.311 kb on - strand, within BWI76_RS18960 at 3932.317 kb on + strand, within BWI76_RS18960 at 3932.326 kb on - strand, within BWI76_RS18960 at 3932.338 kb on + strand, within BWI76_RS18960 at 3932.341 kb on + strand, within BWI76_RS18960 at 3932.346 kb on + strand, within BWI76_RS18960 at 3932.346 kb on + strand, within BWI76_RS18960 at 3932.346 kb on + strand, within BWI76_RS18960 at 3932.346 kb on + strand, within BWI76_RS18960 at 3932.346 kb on + strand, within BWI76_RS18960 at 3932.346 kb on + strand, within BWI76_RS18960 at 3932.354 kb on - strand, within BWI76_RS18960 at 3932.354 kb on - strand, within BWI76_RS18960 at 3932.355 kb on - strand, within BWI76_RS18960 at 3932.444 kb on - strand, within BWI76_RS18960 at 3932.463 kb on - strand, within BWI76_RS18960 at 3932.463 kb on - strand at 3932.480 kb on + strand, within BWI76_RS18960 at 3932.513 kb on - strand, within BWI76_RS18960 at 3932.516 kb on + strand, within BWI76_RS18960 at 3932.516 kb on + strand, within BWI76_RS18960 at 3932.524 kb on - strand, within BWI76_RS18960 at 3932.524 kb on - strand, within BWI76_RS18960 at 3932.524 kb on - strand, within BWI76_RS18960 at 3932.570 kb on + strand, within BWI76_RS18960 at 3932.578 kb on - strand, within BWI76_RS18960 at 3932.585 kb on - strand, within BWI76_RS18960 at 3932.624 kb on + strand, within BWI76_RS18960 at 3932.624 kb on + strand at 3932.629 kb on + strand, within BWI76_RS18960 at 3932.632 kb on - strand, within BWI76_RS18960 at 3932.637 kb on - strand, within BWI76_RS18960
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,930,057 + BWI76_RS18945 0.68 -3.0 3,930,223 - -1.4 3,930,377 + BWI76_RS18950 0.13 -1.8 3,930,426 + BWI76_RS18950 0.20 +0.8 3,930,445 + BWI76_RS18950 0.22 -1.1 3,930,909 + BWI76_RS18950 0.82 -1.8 3,931,121 + BWI76_RS18955 0.13 -1.0 3,931,498 - BWI76_RS18955 0.76 -0.4 3,931,509 + BWI76_RS18955 0.78 -2.4 3,931,517 + BWI76_RS18955 0.79 -2.0 3,931,517 + BWI76_RS18955 0.79 -0.1 3,931,640 - +0.2 3,931,653 - +0.6 3,931,674 + +1.0 3,931,723 - +0.2 3,931,762 + -2.3 3,931,770 - +0.8 3,931,816 - +0.0 3,931,816 - -0.2 3,931,910 - BWI76_RS18960 0.14 +1.0 3,931,936 + BWI76_RS18960 0.16 -0.4 3,932,013 + BWI76_RS18960 0.22 +0.3 3,932,036 + BWI76_RS18960 0.23 -1.6 3,932,045 - BWI76_RS18960 0.24 +1.1 3,932,094 + -0.2 3,932,094 + BWI76_RS18960 0.28 +0.5 3,932,104 - BWI76_RS18960 0.29 +2.1 3,932,112 + BWI76_RS18960 0.29 +0.2 3,932,196 + BWI76_RS18960 0.36 +0.0 3,932,196 + BWI76_RS18960 0.36 -0.7 3,932,196 + BWI76_RS18960 0.36 -2.7 3,932,196 + BWI76_RS18960 0.36 +0.5 3,932,204 - BWI76_RS18960 0.36 -0.1 3,932,204 - BWI76_RS18960 0.36 +0.1 3,932,311 - BWI76_RS18960 0.44 -1.1 3,932,311 - BWI76_RS18960 0.44 -0.1 3,932,317 + BWI76_RS18960 0.45 -0.5 3,932,326 - BWI76_RS18960 0.45 -0.2 3,932,338 + BWI76_RS18960 0.46 +0.2 3,932,341 + BWI76_RS18960 0.47 -0.4 3,932,346 + BWI76_RS18960 0.47 -1.0 3,932,346 + BWI76_RS18960 0.47 -0.8 3,932,346 + BWI76_RS18960 0.47 -2.0 3,932,346 + BWI76_RS18960 0.47 -0.6 3,932,346 + BWI76_RS18960 0.47 -1.4 3,932,346 + BWI76_RS18960 0.47 -2.0 3,932,354 - BWI76_RS18960 0.48 -0.9 3,932,354 - BWI76_RS18960 0.48 +0.2 3,932,355 - BWI76_RS18960 0.48 +0.8 3,932,444 - BWI76_RS18960 0.54 +1.2 3,932,463 - BWI76_RS18960 0.56 +0.5 3,932,463 - +0.4 3,932,480 + BWI76_RS18960 0.57 -2.9 3,932,513 - BWI76_RS18960 0.60 -0.4 3,932,516 + BWI76_RS18960 0.60 -1.6 3,932,516 + BWI76_RS18960 0.60 -3.3 3,932,524 - BWI76_RS18960 0.60 +0.4 3,932,524 - BWI76_RS18960 0.60 +1.8 3,932,524 - BWI76_RS18960 0.60 -2.0 3,932,570 + BWI76_RS18960 0.64 -0.6 3,932,578 - BWI76_RS18960 0.65 +1.2 3,932,585 - BWI76_RS18960 0.65 -0.8 3,932,624 + BWI76_RS18960 0.68 -1.1 3,932,624 + -0.8 3,932,629 + BWI76_RS18960 0.68 -0.0 3,932,632 - BWI76_RS18960 0.69 -0.0 3,932,637 - BWI76_RS18960 0.69 +0.8
Or see this region's nucleotide sequence