Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16895 and BWI76_RS16900 are separated by 74 nucleotides BWI76_RS16900 and BWI76_RS16905 overlap by 4 nucleotides BWI76_RS16905 and BWI76_RS16910 overlap by 8 nucleotides
BWI76_RS16895: BWI76_RS16895 - 3-(3-hydroxy-phenyl)propionate transporter MhpT, at 3,503,327 to 3,504,523
_RS16895
BWI76_RS16900: BWI76_RS16900 - 4-hydroxy-2-oxovalerate aldolase, at 3,504,598 to 3,505,614
_RS16900
BWI76_RS16905: BWI76_RS16905 - acetaldehyde dehydrogenase (acetylating), at 3,505,611 to 3,506,561
_RS16905
BWI76_RS16910: BWI76_RS16910 - 2-keto-4-pentenoate hydratase, at 3,506,554 to 3,507,360
_RS16910
Position (kb) 3504
3505
3506 Strain fitness (log2 ratio) -1
0
1 at 3504.087 kb on + strand, within BWI76_RS16895 at 3504.221 kb on + strand, within BWI76_RS16895 at 3504.460 kb on + strand at 3504.836 kb on + strand, within BWI76_RS16900 at 3505.512 kb on - strand, within BWI76_RS16900 at 3506.110 kb on - strand, within BWI76_RS16905 at 3506.206 kb on + strand, within BWI76_RS16905 at 3506.232 kb on + strand, within BWI76_RS16905 at 3506.287 kb on - strand, within BWI76_RS16905 at 3506.575 kb on - strand at 3506.594 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,504,087 + BWI76_RS16895 0.63 +0.6 3,504,221 + BWI76_RS16895 0.75 +0.4 3,504,460 + +0.4 3,504,836 + BWI76_RS16900 0.23 -0.1 3,505,512 - BWI76_RS16900 0.90 +1.1 3,506,110 - BWI76_RS16905 0.52 -0.3 3,506,206 + BWI76_RS16905 0.63 -0.2 3,506,232 + BWI76_RS16905 0.65 +0.5 3,506,287 - BWI76_RS16905 0.71 -0.7 3,506,575 - -0.3 3,506,594 - +0.7
Or see this region's nucleotide sequence