Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16865 and BWI76_RS16870 are separated by 145 nucleotides BWI76_RS16870 and BWI76_RS16875 are separated by 107 nucleotides BWI76_RS16875 and BWI76_RS16880 are separated by 1 nucleotides
BWI76_RS16865: BWI76_RS16865 - coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase-related flavin-dependent oxidoreductase, at 3,497,494 to 3,498,579
_RS16865
BWI76_RS16870: BWI76_RS16870 - LysR family transcriptional regulator, at 3,498,725 to 3,499,618
_RS16870
BWI76_RS16875: BWI76_RS16875 - putative type I cysteine dioxygenase, at 3,499,726 to 3,500,322
_RS16875
BWI76_RS16880: BWI76_RS16880 - GlpE family protein, at 3,500,324 to 3,501,904
_RS16880
Position (kb)
3498
3499
3500 Strain fitness (log2 ratio)
-1
0
1 at 3497.738 kb on + strand, within BWI76_RS16865 at 3498.029 kb on + strand, within BWI76_RS16865 at 3498.154 kb on + strand, within BWI76_RS16865 at 3498.173 kb on + strand at 3498.181 kb on - strand, within BWI76_RS16865 at 3498.647 kb on - strand at 3498.975 kb on + strand, within BWI76_RS16870 at 3498.976 kb on - strand, within BWI76_RS16870 at 3499.376 kb on - strand, within BWI76_RS16870 at 3499.812 kb on + strand, within BWI76_RS16875 at 3499.991 kb on + strand, within BWI76_RS16875 at 3500.040 kb on - strand, within BWI76_RS16875 at 3500.097 kb on + strand, within BWI76_RS16875 at 3500.156 kb on + strand, within BWI76_RS16875 at 3500.170 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,497,738 + BWI76_RS16865 0.22 -0.4 3,498,029 + BWI76_RS16865 0.49 -1.1 3,498,154 + BWI76_RS16865 0.61 +0.6 3,498,173 + -1.7 3,498,181 - BWI76_RS16865 0.63 -0.6 3,498,647 - +0.3 3,498,975 + BWI76_RS16870 0.28 +1.7 3,498,976 - BWI76_RS16870 0.28 +0.6 3,499,376 - BWI76_RS16870 0.73 +0.4 3,499,812 + BWI76_RS16875 0.14 +0.6 3,499,991 + BWI76_RS16875 0.44 -0.3 3,500,040 - BWI76_RS16875 0.53 +0.3 3,500,097 + BWI76_RS16875 0.62 +0.5 3,500,156 + BWI76_RS16875 0.72 -0.2 3,500,170 - +0.1
Or see this region's nucleotide sequence