Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14570

Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14565 and BWI76_RS14570 are separated by 28 nucleotidesBWI76_RS14570 and BWI76_RS14575 are separated by 88 nucleotidesBWI76_RS14575 and BWI76_RS14580 overlap by 1 nucleotides BWI76_RS14565: BWI76_RS14565 - cysteine hydrolase, at 3,058,393 to 3,058,926 _RS14565 BWI76_RS14570: BWI76_RS14570 - hypothetical protein, at 3,058,955 to 3,059,515 _RS14570 BWI76_RS14575: BWI76_RS14575 - TENA/THI-4 family protein, at 3,059,604 to 3,060,248 _RS14575 BWI76_RS14580: BWI76_RS14580 - hypothetical protein, at 3,060,248 to 3,061,093 _RS14580 Position (kb) 3058 3059 3060Strain fitness (log2 ratio) -2 -1 0 1at 3058.017 kb on + strandat 3058.022 kb on + strandat 3058.025 kb on - strandat 3058.025 kb on - strandat 3058.034 kb on - strandat 3058.071 kb on + strandat 3058.117 kb on - strandat 3058.248 kb on - strandat 3058.257 kb on - strandat 3058.266 kb on + strandat 3058.282 kb on + strandat 3058.318 kb on + strandat 3058.353 kb on - strandat 3058.387 kb on + strandat 3058.596 kb on - strand, within BWI76_RS14565at 3059.281 kb on + strand, within BWI76_RS14570at 3059.324 kb on + strand, within BWI76_RS14570at 3059.353 kb on - strand, within BWI76_RS14570at 3059.370 kb on - strand, within BWI76_RS14570at 3059.548 kb on + strandat 3059.548 kb on + strandat 3059.651 kb on - strandat 3059.768 kb on + strand, within BWI76_RS14575at 3059.982 kb on - strand, within BWI76_RS14575at 3060.294 kb on + strandat 3060.335 kb on - strand, within BWI76_RS14580at 3060.403 kb on + strand, within BWI76_RS14580at 3060.411 kb on - strand, within BWI76_RS14580at 3060.477 kb on + strand, within BWI76_RS14580

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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3,058,017 + -0.1
3,058,022 + -2.1
3,058,025 - +0.7
3,058,025 - +1.6
3,058,034 - -0.0
3,058,071 + -0.2
3,058,117 - +0.5
3,058,248 - -1.3
3,058,257 - +0.9
3,058,266 + +0.7
3,058,282 + -0.1
3,058,318 + +0.6
3,058,353 - -2.3
3,058,387 + +0.9
3,058,596 - BWI76_RS14565 0.38 -2.6
3,059,281 + BWI76_RS14570 0.58 -0.3
3,059,324 + BWI76_RS14570 0.66 -2.7
3,059,353 - BWI76_RS14570 0.71 -1.7
3,059,370 - BWI76_RS14570 0.74 -0.8
3,059,548 + +0.4
3,059,548 + +0.3
3,059,651 - +0.6
3,059,768 + BWI76_RS14575 0.25 -1.2
3,059,982 - BWI76_RS14575 0.59 +0.4
3,060,294 + +0.1
3,060,335 - BWI76_RS14580 0.10 +0.2
3,060,403 + BWI76_RS14580 0.18 -0.2
3,060,411 - BWI76_RS14580 0.19 +0.1
3,060,477 + BWI76_RS14580 0.27 -0.9

Or see this region's nucleotide sequence