Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14065

Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14060 and BWI76_RS14065 are separated by 338 nucleotidesBWI76_RS14065 and BWI76_RS14070 are separated by 5 nucleotidesBWI76_RS14070 and BWI76_RS14075 are separated by 17 nucleotides BWI76_RS14060: BWI76_RS14060 - secretory lipase, at 2,938,480 to 2,939,622 _RS14060 BWI76_RS14065: BWI76_RS14065 - hypothetical protein, at 2,939,961 to 2,940,386 _RS14065 BWI76_RS14070: BWI76_RS14070 - ECF subfamily RNA polymerase sigma-24 subunit, at 2,940,392 to 2,940,952 _RS14070 BWI76_RS14075: BWI76_RS14075 - TetR family transcriptional regulator, at 2,940,970 to 2,941,605 _RS14075 Position (kb) 2939 2940 2941Strain fitness (log2 ratio) -2 -1 0 1at 2939.004 kb on + strand, within BWI76_RS14060at 2939.229 kb on + strand, within BWI76_RS14060at 2939.381 kb on - strand, within BWI76_RS14060at 2939.675 kb on - strandat 2939.823 kb on - strandat 2939.923 kb on - strandat 2939.923 kb on - strandat 2939.925 kb on + strandat 2939.933 kb on - strandat 2940.146 kb on - strand, within BWI76_RS14065at 2940.253 kb on + strand, within BWI76_RS14065at 2940.261 kb on - strand, within BWI76_RS14065at 2940.760 kb on - strand, within BWI76_RS14070at 2941.102 kb on + strand, within BWI76_RS14075at 2941.246 kb on - strand, within BWI76_RS14075

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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2,939,004 + BWI76_RS14060 0.46 +0.8
2,939,229 + BWI76_RS14060 0.66 -0.3
2,939,381 - BWI76_RS14060 0.79 +0.5
2,939,675 - +1.2
2,939,823 - -0.3
2,939,923 - +0.8
2,939,923 - +0.5
2,939,925 + -0.5
2,939,933 - -1.1
2,940,146 - BWI76_RS14065 0.43 -2.2
2,940,253 + BWI76_RS14065 0.69 -1.6
2,940,261 - BWI76_RS14065 0.70 -0.2
2,940,760 - BWI76_RS14070 0.66 +1.5
2,941,102 + BWI76_RS14075 0.21 -1.7
2,941,246 - BWI76_RS14075 0.43 +0.7

Or see this region's nucleotide sequence