Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13470 and BWI76_RS13475 overlap by 4 nucleotides BWI76_RS13475 and BWI76_RS13480 are separated by 276 nucleotides BWI76_RS13480 and BWI76_RS13485 are separated by 65 nucleotides
BWI76_RS13470: BWI76_RS13470 - transporter, at 2,809,041 to 2,809,490
_RS13470
BWI76_RS13475: BWI76_RS13475 - N-acetyltransferase, at 2,809,487 to 2,810,005
_RS13475
BWI76_RS13480: BWI76_RS13480 - XRE family transcriptional regulator, at 2,810,282 to 2,810,857
_RS13480
BWI76_RS13485: BWI76_RS13485 - NADP-dependent oxidoreductase, at 2,810,923 to 2,811,960
_RS13485
Position (kb)
2810
2811 Strain fitness (log2 ratio)
-1
0
1 at 2809.585 kb on + strand at 2809.723 kb on - strand, within BWI76_RS13475 at 2810.105 kb on + strand at 2810.768 kb on - strand, within BWI76_RS13480 at 2811.278 kb on + strand, within BWI76_RS13485 at 2811.343 kb on - strand, within BWI76_RS13485 at 2811.413 kb on - strand, within BWI76_RS13485 at 2811.521 kb on - strand, within BWI76_RS13485
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,809,585 + +1.1 2,809,723 - BWI76_RS13475 0.45 +1.2 2,810,105 + +0.5 2,810,768 - BWI76_RS13480 0.84 +1.1 2,811,278 + BWI76_RS13485 0.34 -1.1 2,811,343 - BWI76_RS13485 0.40 -0.3 2,811,413 - BWI76_RS13485 0.47 -0.4 2,811,521 - BWI76_RS13485 0.58 +0.6
Or see this region's nucleotide sequence