Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12855 and BWI76_RS12860 overlap by 4 nucleotides BWI76_RS12860 and BWI76_RS12865 overlap by 8 nucleotides BWI76_RS12865 and BWI76_RS12870 are separated by 23 nucleotides BWI76_RS12870 and BWI76_RS12875 are separated by 159 nucleotides
BWI76_RS12855: BWI76_RS12855 - enamine deaminase RidA, at 2,679,755 to 2,680,189
_RS12855
BWI76_RS12860: BWI76_RS12860 - hypothetical protein, at 2,680,186 to 2,680,860
_RS12860
BWI76_RS12865: BWI76_RS12865 - acetylornithine deacetylase, at 2,680,853 to 2,682,022
_RS12865
BWI76_RS12870: BWI76_RS12870 - putative phytanoyl-CoA dioxygenase, at 2,682,046 to 2,682,861
_RS12870
BWI76_RS12875: BWI76_RS12875 - bifunctional deaminase-reductase, at 2,683,021 to 2,683,557
_RS12875
Position (kb)
2680
2681
2682
2683 Strain fitness (log2 ratio)
-2
-1
0
1 at 2680.044 kb on + strand, within BWI76_RS12855 at 2680.303 kb on + strand, within BWI76_RS12860 at 2680.518 kb on - strand, within BWI76_RS12860 at 2681.019 kb on - strand, within BWI76_RS12865 at 2681.110 kb on - strand, within BWI76_RS12865 at 2681.268 kb on + strand, within BWI76_RS12865 at 2681.368 kb on - strand, within BWI76_RS12865 at 2681.368 kb on - strand, within BWI76_RS12865 at 2681.518 kb on - strand, within BWI76_RS12865 at 2681.906 kb on + strand at 2681.921 kb on - strand at 2682.117 kb on + strand at 2682.117 kb on + strand at 2682.205 kb on - strand, within BWI76_RS12870 at 2682.302 kb on + strand, within BWI76_RS12870 at 2682.418 kb on + strand, within BWI76_RS12870 at 2682.797 kb on + strand at 2682.911 kb on + strand at 2683.019 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,680,044 + BWI76_RS12855 0.66 -2.1 2,680,303 + BWI76_RS12860 0.17 -0.6 2,680,518 - BWI76_RS12860 0.49 +0.5 2,681,019 - BWI76_RS12865 0.14 -0.4 2,681,110 - BWI76_RS12865 0.22 +0.1 2,681,268 + BWI76_RS12865 0.35 +1.0 2,681,368 - BWI76_RS12865 0.44 +1.6 2,681,368 - BWI76_RS12865 0.44 +1.7 2,681,518 - BWI76_RS12865 0.57 -2.3 2,681,906 + +0.5 2,681,921 - +0.1 2,682,117 + +0.6 2,682,117 + +1.4 2,682,205 - BWI76_RS12870 0.19 +0.2 2,682,302 + BWI76_RS12870 0.31 +0.8 2,682,418 + BWI76_RS12870 0.46 +0.8 2,682,797 + +0.3 2,682,911 + -1.2 2,683,019 + -1.6
Or see this region's nucleotide sequence