Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12460 and BWI76_RS12465 are separated by 19 nucleotides BWI76_RS12465 and BWI76_RS12470 are separated by 33 nucleotides BWI76_RS12470 and BWI76_RS12475 are separated by 100 nucleotides BWI76_RS12475 and BWI76_RS12480 overlap by 4 nucleotides
BWI76_RS12460: BWI76_RS12460 - envelope stress response membrane protein PspC, at 2,603,605 to 2,603,964
_RS12460
BWI76_RS12465: BWI76_RS12465 - phage shock protein D, at 2,603,984 to 2,604,214
_RS12465
BWI76_RS12470: BWI76_RS12470 - thiosulfate sulfurtransferase PspE, at 2,604,248 to 2,604,562
_RS12470
BWI76_RS12475: BWI76_RS12475 - hypothetical protein, at 2,604,663 to 2,606,060
_RS12475
BWI76_RS12480: BWI76_RS12480 - TIGR01620 family protein, at 2,606,057 to 2,607,118
_RS12480
Position (kb)
2604
2605
2606
2607 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2603.698 kb on - strand, within BWI76_RS12460 at 2603.731 kb on - strand, within BWI76_RS12460 at 2604.075 kb on - strand, within BWI76_RS12465 at 2604.208 kb on + strand at 2604.307 kb on + strand, within BWI76_RS12470 at 2604.762 kb on + strand at 2604.764 kb on + strand at 2604.787 kb on - strand at 2604.787 kb on - strand at 2604.931 kb on - strand, within BWI76_RS12475 at 2605.111 kb on - strand, within BWI76_RS12475 at 2605.161 kb on - strand, within BWI76_RS12475 at 2605.223 kb on - strand, within BWI76_RS12475 at 2605.375 kb on + strand, within BWI76_RS12475 at 2605.385 kb on + strand, within BWI76_RS12475 at 2605.605 kb on - strand, within BWI76_RS12475 at 2605.670 kb on + strand, within BWI76_RS12475 at 2605.791 kb on - strand, within BWI76_RS12475 at 2605.869 kb on - strand, within BWI76_RS12475 at 2605.934 kb on - strand at 2606.225 kb on - strand, within BWI76_RS12480 at 2606.447 kb on - strand, within BWI76_RS12480 at 2606.567 kb on + strand, within BWI76_RS12480 at 2606.585 kb on - strand, within BWI76_RS12480 at 2606.804 kb on - strand, within BWI76_RS12480 at 2606.925 kb on + strand, within BWI76_RS12480
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,603,698 - BWI76_RS12460 0.26 +3.0 2,603,731 - BWI76_RS12460 0.35 +0.2 2,604,075 - BWI76_RS12465 0.39 +0.5 2,604,208 + +0.3 2,604,307 + BWI76_RS12470 0.19 +0.6 2,604,762 + +1.6 2,604,764 + -2.6 2,604,787 - -1.5 2,604,787 - -0.3 2,604,931 - BWI76_RS12475 0.19 -0.4 2,605,111 - BWI76_RS12475 0.32 +0.7 2,605,161 - BWI76_RS12475 0.36 +1.6 2,605,223 - BWI76_RS12475 0.40 +0.6 2,605,375 + BWI76_RS12475 0.51 +0.0 2,605,385 + BWI76_RS12475 0.52 +0.7 2,605,605 - BWI76_RS12475 0.67 +1.3 2,605,670 + BWI76_RS12475 0.72 +1.6 2,605,791 - BWI76_RS12475 0.81 +0.7 2,605,869 - BWI76_RS12475 0.86 +0.8 2,605,934 - -0.0 2,606,225 - BWI76_RS12480 0.16 -0.8 2,606,447 - BWI76_RS12480 0.37 -1.5 2,606,567 + BWI76_RS12480 0.48 +0.3 2,606,585 - BWI76_RS12480 0.50 +0.7 2,606,804 - BWI76_RS12480 0.70 +0.3 2,606,925 + BWI76_RS12480 0.82 +0.1
Or see this region's nucleotide sequence