Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12075 and BWI76_RS12080 are separated by 140 nucleotides BWI76_RS12080 and BWI76_RS12085 are separated by 2 nucleotides BWI76_RS12085 and BWI76_RS12090 are separated by 11 nucleotides BWI76_RS12090 and BWI76_RS12095 are separated by 332 nucleotides
BWI76_RS12075: BWI76_RS12075 - regulator, at 2,537,846 to 2,538,406
_RS12075
BWI76_RS12080: BWI76_RS12080 - hypothetical protein, at 2,538,547 to 2,538,777
_RS12080
BWI76_RS12085: BWI76_RS12085 - regulatory protein CII from bacteriophage origin, at 2,538,780 to 2,539,316
_RS12085
BWI76_RS12090: BWI76_RS12090 - HNH endonuclease, at 2,539,328 to 2,539,831
_RS12090
BWI76_RS12095: BWI76_RS12095 - hypothetical protein, at 2,540,164 to 2,541,084
_RS12095
Position (kb)
2539
2540 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2538.634 kb on - strand, within BWI76_RS12080 at 2538.687 kb on + strand, within BWI76_RS12080 at 2538.703 kb on - strand, within BWI76_RS12080 at 2538.778 kb on - strand at 2538.860 kb on + strand, within BWI76_RS12085 at 2538.880 kb on - strand, within BWI76_RS12085 at 2538.900 kb on - strand, within BWI76_RS12085 at 2538.983 kb on + strand, within BWI76_RS12085 at 2538.983 kb on + strand, within BWI76_RS12085 at 2538.991 kb on - strand, within BWI76_RS12085 at 2539.183 kb on + strand, within BWI76_RS12085 at 2539.188 kb on + strand, within BWI76_RS12085 at 2539.258 kb on + strand, within BWI76_RS12085 at 2539.273 kb on - strand at 2539.278 kb on + strand at 2539.302 kb on - strand at 2539.339 kb on - strand at 2539.339 kb on - strand at 2539.500 kb on - strand, within BWI76_RS12090 at 2539.558 kb on + strand, within BWI76_RS12090 at 2539.566 kb on - strand, within BWI76_RS12090 at 2539.723 kb on + strand at 2539.890 kb on - strand at 2539.908 kb on - strand at 2540.163 kb on - strand at 2540.163 kb on - strand at 2540.166 kb on + strand at 2540.505 kb on + strand, within BWI76_RS12095 at 2540.758 kb on + strand, within BWI76_RS12095 at 2540.766 kb on + strand, within BWI76_RS12095 at 2540.766 kb on + strand, within BWI76_RS12095 at 2540.774 kb on - strand, within BWI76_RS12095 at 2540.774 kb on - strand, within BWI76_RS12095
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,538,634 - BWI76_RS12080 0.38 +0.1 2,538,687 + BWI76_RS12080 0.61 +0.9 2,538,703 - BWI76_RS12080 0.68 -0.6 2,538,778 - +0.1 2,538,860 + BWI76_RS12085 0.15 +1.3 2,538,880 - BWI76_RS12085 0.19 +0.3 2,538,900 - BWI76_RS12085 0.22 -1.4 2,538,983 + BWI76_RS12085 0.38 -0.7 2,538,983 + BWI76_RS12085 0.38 -0.1 2,538,991 - BWI76_RS12085 0.39 +0.9 2,539,183 + BWI76_RS12085 0.75 -0.3 2,539,188 + BWI76_RS12085 0.76 +1.3 2,539,258 + BWI76_RS12085 0.89 +1.3 2,539,273 - -0.8 2,539,278 + -3.4 2,539,302 - +2.0 2,539,339 - -3.0 2,539,339 - -0.7 2,539,500 - BWI76_RS12090 0.34 +0.3 2,539,558 + BWI76_RS12090 0.46 -4.1 2,539,566 - BWI76_RS12090 0.47 -1.9 2,539,723 + +1.3 2,539,890 - -0.7 2,539,908 - -0.9 2,540,163 - +0.3 2,540,163 - -1.2 2,540,166 + -0.6 2,540,505 + BWI76_RS12095 0.37 -0.4 2,540,758 + BWI76_RS12095 0.64 +0.4 2,540,766 + BWI76_RS12095 0.65 +0.2 2,540,766 + BWI76_RS12095 0.65 +0.7 2,540,774 - BWI76_RS12095 0.66 -2.4 2,540,774 - BWI76_RS12095 0.66 +0.2
Or see this region's nucleotide sequence