Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11780 and BWI76_RS11785 are separated by 130 nucleotides BWI76_RS11785 and BWI76_RS11790 are separated by 32 nucleotides BWI76_RS11790 and BWI76_RS11795 are separated by 99 nucleotides
BWI76_RS11780: BWI76_RS11780 - 2-deoxyglucose-6-phosphatase, at 2,478,445 to 2,479,113
_RS11780
BWI76_RS11785: BWI76_RS11785 - hypothetical protein, at 2,479,244 to 2,479,780
_RS11785
BWI76_RS11790: BWI76_RS11790 - acyl-CoA thioesterase, at 2,479,813 to 2,480,211
_RS11790
BWI76_RS11795: BWI76_RS11795 - TonB-dependent siderophore receptor, at 2,480,311 to 2,482,491
_RS11795
Position (kb)
2479
2480
2481 Strain fitness (log2 ratio)
-1
0
1 at 2478.926 kb on + strand, within BWI76_RS11780 at 2479.249 kb on - strand at 2479.249 kb on - strand at 2479.401 kb on + strand, within BWI76_RS11785 at 2480.138 kb on + strand, within BWI76_RS11790 at 2480.574 kb on - strand, within BWI76_RS11795 at 2480.610 kb on + strand, within BWI76_RS11795 at 2480.618 kb on - strand, within BWI76_RS11795 at 2481.066 kb on - strand, within BWI76_RS11795 at 2481.106 kb on + strand, within BWI76_RS11795 at 2481.114 kb on - strand, within BWI76_RS11795
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,478,926 + BWI76_RS11780 0.72 -0.1 2,479,249 - +1.2 2,479,249 - -1.0 2,479,401 + BWI76_RS11785 0.29 +1.2 2,480,138 + BWI76_RS11790 0.81 +1.2 2,480,574 - BWI76_RS11795 0.12 +1.0 2,480,610 + BWI76_RS11795 0.14 +0.1 2,480,618 - BWI76_RS11795 0.14 +1.0 2,481,066 - BWI76_RS11795 0.35 +0.2 2,481,106 + BWI76_RS11795 0.36 -0.1 2,481,114 - BWI76_RS11795 0.37 +0.6
Or see this region's nucleotide sequence