Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10950

Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10935 and BWI76_RS10940 are separated by 66 nucleotidesBWI76_RS10940 and BWI76_RS10945 are separated by 0 nucleotidesBWI76_RS10945 and BWI76_RS10950 are separated by 79 nucleotidesBWI76_RS10950 and BWI76_RS10955 overlap by 95 nucleotidesBWI76_RS10955 and BWI76_RS10960 overlap by 38 nucleotides BWI76_RS10935: BWI76_RS10935 - glucan biosynthesis glucosyltransferase H, at 2,309,147 to 2,311,675 _RS10935 BWI76_RS10940: BWI76_RS10940 - YceK/YidQ family lipoprotein, at 2,311,742 to 2,311,969 _RS10940 BWI76_RS10945: BWI76_RS10945 - SecY/SecA suppressor protein, at 2,311,970 to 2,312,356 _RS10945 BWI76_RS10950: BWI76_RS10950 - MFS transporter, at 2,312,436 to 2,313,686 _RS10950 BWI76_RS10955: BWI76_RS10955 - hypothetical protein, at 2,313,592 to 2,313,899 _RS10955 BWI76_RS10960: BWI76_RS10960 - lipid A biosynthesis lauroyl acyltransferase, at 2,313,862 to 2,314,782 _RS10960 Position (kb) 2312 2313 2314Strain fitness (log2 ratio) -2 -1 0 1at 2312.304 kb on - strand, within BWI76_RS10945at 2312.539 kb on - strandat 2313.263 kb on + strand, within BWI76_RS10950at 2313.339 kb on + strand, within BWI76_RS10950at 2313.356 kb on + strand, within BWI76_RS10950at 2313.769 kb on + strand, within BWI76_RS10955at 2313.844 kb on - strand, within BWI76_RS10955

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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2,312,304 - BWI76_RS10945 0.86 +0.1
2,312,539 - +0.3
2,313,263 + BWI76_RS10950 0.66 +0.0
2,313,339 + BWI76_RS10950 0.72 -0.8
2,313,356 + BWI76_RS10950 0.74 -2.2
2,313,769 + BWI76_RS10955 0.57 +0.6
2,313,844 - BWI76_RS10955 0.82 -0.1

Or see this region's nucleotide sequence