Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10540 and BWI76_RS10545 are separated by 227 nucleotides BWI76_RS10545 and BWI76_RS10550 overlap by 1 nucleotides BWI76_RS10550 and BWI76_RS10555 overlap by 4 nucleotides BWI76_RS10555 and BWI76_RS10560 are separated by 9 nucleotides
BWI76_RS10540: BWI76_RS10540 - allantoinase, at 2,226,929 to 2,227,861
_RS10540
BWI76_RS10545: BWI76_RS10545 - hypothetical protein, at 2,228,089 to 2,228,472
_RS10545
BWI76_RS10550: BWI76_RS10550 - AtzE family amidohydrolase, at 2,228,472 to 2,229,869
_RS10550
BWI76_RS10555: BWI76_RS10555 - hypothetical protein, at 2,229,866 to 2,230,054
_RS10555
BWI76_RS10560: BWI76_RS10560 - gamma-glutamyltransferase, at 2,230,064 to 2,231,647
_RS10560
Position (kb)
2228
2229
2230 Strain fitness (log2 ratio)
-2
-1
0
1 at 2227.618 kb on - strand, within BWI76_RS10540 at 2227.644 kb on - strand, within BWI76_RS10540 at 2227.667 kb on - strand, within BWI76_RS10540 at 2227.754 kb on - strand, within BWI76_RS10540 at 2227.888 kb on + strand at 2227.909 kb on - strand at 2228.048 kb on + strand at 2228.077 kb on - strand at 2228.117 kb on - strand at 2228.716 kb on - strand, within BWI76_RS10550 at 2229.024 kb on + strand, within BWI76_RS10550 at 2229.082 kb on + strand, within BWI76_RS10550 at 2229.087 kb on + strand, within BWI76_RS10550 at 2229.203 kb on + strand, within BWI76_RS10550 at 2230.028 kb on - strand, within BWI76_RS10555 at 2230.180 kb on + strand at 2230.188 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,227,618 - BWI76_RS10540 0.74 +0.3 2,227,644 - BWI76_RS10540 0.77 -0.3 2,227,667 - BWI76_RS10540 0.79 +0.1 2,227,754 - BWI76_RS10540 0.88 +0.8 2,227,888 + +0.4 2,227,909 - +1.4 2,228,048 + -0.9 2,228,077 - +0.3 2,228,117 - -0.0 2,228,716 - BWI76_RS10550 0.17 +0.3 2,229,024 + BWI76_RS10550 0.39 +0.1 2,229,082 + BWI76_RS10550 0.44 +1.2 2,229,087 + BWI76_RS10550 0.44 +1.7 2,229,203 + BWI76_RS10550 0.52 +0.6 2,230,028 - BWI76_RS10555 0.86 -1.9 2,230,180 + +0.8 2,230,188 - -0.5
Or see this region's nucleotide sequence