Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09460 and BWI76_RS09465 are separated by 57 nucleotides BWI76_RS09465 and BWI76_RS09470 are separated by 10 nucleotides BWI76_RS09470 and BWI76_RS09475 overlap by 4 nucleotides
BWI76_RS09460: BWI76_RS09460 - spermidine/putrescine ABC transporter substrate-binding protein PotF, at 1,980,576 to 1,981,688
_RS09460
BWI76_RS09465: BWI76_RS09465 - polyamine ABC transporter ATP-binding protein, at 1,981,746 to 1,982,924
_RS09465
BWI76_RS09470: BWI76_RS09470 - putrescine ABC transporter permease, at 1,982,935 to 1,983,888
_RS09470
BWI76_RS09475: BWI76_RS09475 - putrescine ABC transporter permease PotI, at 1,983,885 to 1,984,730
_RS09475
Position (kb)
1981
1982
1983 Strain fitness (log2 ratio)
-2
-1
0
1 at 1980.790 kb on - strand, within BWI76_RS09460 at 1980.816 kb on - strand, within BWI76_RS09460 at 1980.864 kb on - strand, within BWI76_RS09460 at 1981.028 kb on + strand, within BWI76_RS09460 at 1981.036 kb on - strand, within BWI76_RS09460 at 1981.504 kb on - strand, within BWI76_RS09460 at 1982.027 kb on - strand, within BWI76_RS09465 at 1982.245 kb on - strand, within BWI76_RS09465 at 1982.359 kb on - strand, within BWI76_RS09465 at 1982.535 kb on - strand, within BWI76_RS09465 at 1982.595 kb on + strand, within BWI76_RS09465 at 1982.603 kb on - strand, within BWI76_RS09465 at 1982.603 kb on - strand, within BWI76_RS09465 at 1982.613 kb on - strand, within BWI76_RS09465 at 1982.724 kb on + strand at 1982.869 kb on - strand at 1983.548 kb on - strand, within BWI76_RS09470 at 1983.640 kb on - strand, within BWI76_RS09470 at 1983.739 kb on + strand, within BWI76_RS09470 at 1983.802 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 1,980,790 - BWI76_RS09460 0.19 +0.3 1,980,816 - BWI76_RS09460 0.22 +1.5 1,980,864 - BWI76_RS09460 0.26 +1.0 1,981,028 + BWI76_RS09460 0.41 -0.5 1,981,036 - BWI76_RS09460 0.41 -1.6 1,981,504 - BWI76_RS09460 0.83 +0.5 1,982,027 - BWI76_RS09465 0.24 +1.3 1,982,245 - BWI76_RS09465 0.42 +1.0 1,982,359 - BWI76_RS09465 0.52 +1.0 1,982,535 - BWI76_RS09465 0.67 -0.4 1,982,595 + BWI76_RS09465 0.72 +0.2 1,982,603 - BWI76_RS09465 0.73 +1.2 1,982,603 - BWI76_RS09465 0.73 +0.8 1,982,613 - BWI76_RS09465 0.74 -1.3 1,982,724 + +1.2 1,982,869 - -0.4 1,983,548 - BWI76_RS09470 0.64 -2.7 1,983,640 - BWI76_RS09470 0.74 -0.1 1,983,739 + BWI76_RS09470 0.84 +0.8 1,983,802 - -0.2
Or see this region's nucleotide sequence