Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09135

Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09125 and BWI76_RS09130 are separated by 376 nucleotidesBWI76_RS09130 and BWI76_RS09135 are separated by 104 nucleotidesBWI76_RS09135 and BWI76_RS09140 are separated by 2 nucleotidesBWI76_RS09140 and BWI76_RS09145 are separated by 257 nucleotides BWI76_RS09125: BWI76_RS09125 - MFS transporter, at 1,927,852 to 1,929,096 _RS09125 BWI76_RS09130: BWI76_RS09130 - biofilm formation regulatory protein BssR, at 1,929,473 to 1,929,856 _RS09130 BWI76_RS09135: BWI76_RS09135 - oxidoreductase, at 1,929,961 to 1,931,073 _RS09135 BWI76_RS09140: BWI76_RS09140 - glutathione S-transferase, at 1,931,076 to 1,931,702 _RS09140 BWI76_RS09145: BWI76_RS09145 - serine-type D-Ala-D-Ala carboxypeptidase, at 1,931,960 to 1,933,162 _RS09145 Position (kb) 1929 1930 1931 1932Strain fitness (log2 ratio) -2 -1 0 1 2at 1929.039 kb on - strandat 1929.105 kb on + strandat 1929.126 kb on - strandat 1929.476 kb on + strandat 1929.495 kb on - strandat 1929.619 kb on + strand, within BWI76_RS09130at 1929.740 kb on + strand, within BWI76_RS09130at 1929.748 kb on - strand, within BWI76_RS09130at 1929.929 kb on - strandat 1930.520 kb on - strand, within BWI76_RS09135at 1930.603 kb on + strand, within BWI76_RS09135at 1930.603 kb on + strand, within BWI76_RS09135at 1930.611 kb on - strand, within BWI76_RS09135at 1930.730 kb on + strand, within BWI76_RS09135at 1931.042 kb on - strandat 1931.229 kb on + strand, within BWI76_RS09140at 1931.268 kb on + strand, within BWI76_RS09140at 1931.352 kb on - strand, within BWI76_RS09140at 1931.352 kb on - strand, within BWI76_RS09140at 1931.400 kb on - strand, within BWI76_RS09140at 1931.881 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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1,929,039 - +0.4
1,929,105 + -1.1
1,929,126 - -2.6
1,929,476 + +0.5
1,929,495 - -2.0
1,929,619 + BWI76_RS09130 0.38 +1.7
1,929,740 + BWI76_RS09130 0.70 +1.1
1,929,748 - BWI76_RS09130 0.72 -0.8
1,929,929 - +0.4
1,930,520 - BWI76_RS09135 0.50 -0.8
1,930,603 + BWI76_RS09135 0.58 +0.9
1,930,603 + BWI76_RS09135 0.58 +1.8
1,930,611 - BWI76_RS09135 0.58 +0.5
1,930,730 + BWI76_RS09135 0.69 +0.5
1,931,042 - +0.2
1,931,229 + BWI76_RS09140 0.24 +0.2
1,931,268 + BWI76_RS09140 0.31 +0.5
1,931,352 - BWI76_RS09140 0.44 +0.2
1,931,352 - BWI76_RS09140 0.44 +0.2
1,931,400 - BWI76_RS09140 0.52 +0.4
1,931,881 + +0.2

Or see this region's nucleotide sequence