Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08520 and BWI76_RS08525 are separated by 3 nucleotides BWI76_RS08525 and BWI76_RS08530 are separated by 105 nucleotides BWI76_RS08530 and BWI76_RS08535 are separated by 128 nucleotides
BWI76_RS08520: BWI76_RS08520 - colicin transporter TolQ, at 1,803,797 to 1,804,480
_RS08520
BWI76_RS08525: BWI76_RS08525 - colicin transporter, at 1,804,484 to 1,804,912
_RS08525
BWI76_RS08530: BWI76_RS08530 - protein TolA, at 1,805,018 to 1,806,355
_RS08530
BWI76_RS08535: BWI76_RS08535 - translocation protein TolB, at 1,806,484 to 1,807,776
_RS08535
Position (kb) 1805
1806
1807 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1804.358 kb on + strand, within BWI76_RS08520 at 1804.368 kb on + strand, within BWI76_RS08520 at 1804.421 kb on - strand at 1804.433 kb on + strand at 1804.486 kb on + strand at 1804.491 kb on + strand at 1804.546 kb on + strand, within BWI76_RS08525 at 1804.697 kb on + strand, within BWI76_RS08525 at 1804.753 kb on + strand, within BWI76_RS08525 at 1805.247 kb on + strand, within BWI76_RS08530 at 1806.168 kb on - strand, within BWI76_RS08530 at 1806.468 kb on + strand at 1806.476 kb on - strand at 1806.619 kb on + strand, within BWI76_RS08535 at 1806.763 kb on - strand at 1807.007 kb on - strand, within BWI76_RS08535 at 1807.291 kb on - strand, within BWI76_RS08535
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 1,804,358 + BWI76_RS08520 0.82 +0.1 1,804,368 + BWI76_RS08520 0.83 -0.5 1,804,421 - +0.1 1,804,433 + -1.4 1,804,486 + -2.1 1,804,491 + -2.5 1,804,546 + BWI76_RS08525 0.14 -2.1 1,804,697 + BWI76_RS08525 0.50 +1.1 1,804,753 + BWI76_RS08525 0.63 -0.5 1,805,247 + BWI76_RS08530 0.17 -2.4 1,806,168 - BWI76_RS08530 0.86 -3.2 1,806,468 + +0.6 1,806,476 - +1.1 1,806,619 + BWI76_RS08535 0.10 -1.7 1,806,763 - +0.1 1,807,007 - BWI76_RS08535 0.40 -0.9 1,807,291 - BWI76_RS08535 0.62 +1.1
Or see this region's nucleotide sequence