Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07975 and BWI76_RS07980 are separated by 195 nucleotides BWI76_RS07980 and BWI76_RS07985 are separated by 58 nucleotides BWI76_RS07985 and BWI76_RS07990 are separated by 91 nucleotides
BWI76_RS07975: BWI76_RS07975 - phospholipid:lipid A palmitoyltransferase, at 1,693,373 to 1,693,942
_RS07975
BWI76_RS07980: BWI76_RS07980 - cold-shock protein, at 1,694,138 to 1,694,347
_RS07980
BWI76_RS07985: BWI76_RS07985 - fluoride ion transporter CrcB, at 1,694,406 to 1,694,789
_RS07985
BWI76_RS07990: BWI76_RS07990 - carbon-nitrogen hydrolase, at 1,694,881 to 1,695,669
_RS07990
Position (kb)
1694
1695 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1693.428 kb on - strand at 1693.554 kb on - strand, within BWI76_RS07975 at 1693.554 kb on - strand, within BWI76_RS07975 at 1693.668 kb on - strand, within BWI76_RS07975 at 1693.703 kb on - strand, within BWI76_RS07975 at 1693.729 kb on + strand, within BWI76_RS07975 at 1693.729 kb on + strand, within BWI76_RS07975 at 1693.886 kb on - strand at 1693.917 kb on - strand at 1694.157 kb on + strand at 1694.674 kb on - strand, within BWI76_RS07985
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 1,693,428 - -0.6 1,693,554 - BWI76_RS07975 0.32 -0.2 1,693,554 - BWI76_RS07975 0.32 +1.1 1,693,668 - BWI76_RS07975 0.52 +2.7 1,693,703 - BWI76_RS07975 0.58 -0.2 1,693,729 + BWI76_RS07975 0.62 +0.5 1,693,729 + BWI76_RS07975 0.62 -0.2 1,693,886 - -0.7 1,693,917 - +0.4 1,694,157 + -0.1 1,694,674 - BWI76_RS07985 0.70 -3.3
Or see this region's nucleotide sequence