Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07630 and BWI76_RS07635 are separated by 222 nucleotides BWI76_RS07635 and BWI76_RS07640 are separated by 193 nucleotides BWI76_RS07640 and BWI76_RS07645 are separated by 199 nucleotides
BWI76_RS07630: BWI76_RS07630 - high-affinity choline transporter BetT, at 1,615,343 to 1,617,376
_RS07630
BWI76_RS07635: BWI76_RS07635 - Hok/gef cell toxic protein, at 1,617,599 to 1,617,751
_RS07635
BWI76_RS07640: BWI76_RS07640 - short chain dehydrogenase, at 1,617,945 to 1,618,730
_RS07640
BWI76_RS07645: BWI76_RS07645 - short-chain dehydrogenase/reductase, at 1,618,930 to 1,619,727
_RS07645
Position (kb)
1617
1618
1619 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1616.963 kb on + strand, within BWI76_RS07630 at 1617.084 kb on + strand, within BWI76_RS07630 at 1617.371 kb on + strand at 1617.637 kb on + strand, within BWI76_RS07635 at 1617.647 kb on - strand, within BWI76_RS07635 at 1617.647 kb on - strand, within BWI76_RS07635 at 1617.660 kb on + strand at 1617.668 kb on - strand, within BWI76_RS07635 at 1617.668 kb on - strand, within BWI76_RS07635 at 1617.800 kb on + strand at 1617.807 kb on - strand at 1617.890 kb on + strand at 1617.890 kb on - strand at 1618.235 kb on - strand, within BWI76_RS07640 at 1618.235 kb on - strand, within BWI76_RS07640 at 1618.272 kb on + strand, within BWI76_RS07640 at 1618.777 kb on - strand at 1619.180 kb on - strand, within BWI76_RS07645 at 1619.468 kb on - strand, within BWI76_RS07645
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 1,616,963 + BWI76_RS07630 0.80 -1.0 1,617,084 + BWI76_RS07630 0.86 +0.6 1,617,371 + +0.4 1,617,637 + BWI76_RS07635 0.25 +0.5 1,617,647 - BWI76_RS07635 0.31 -0.6 1,617,647 - BWI76_RS07635 0.31 -1.2 1,617,660 + +1.1 1,617,668 - BWI76_RS07635 0.45 -0.7 1,617,668 - BWI76_RS07635 0.45 -0.5 1,617,800 + -0.9 1,617,807 - -0.2 1,617,890 + +1.0 1,617,890 - -0.5 1,618,235 - BWI76_RS07640 0.37 -2.0 1,618,235 - BWI76_RS07640 0.37 -0.5 1,618,272 + BWI76_RS07640 0.42 -0.7 1,618,777 - -0.6 1,619,180 - BWI76_RS07645 0.31 +0.6 1,619,468 - BWI76_RS07645 0.67 +2.1
Or see this region's nucleotide sequence