Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS06030

Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS06025 and BWI76_RS06030 overlap by 14 nucleotidesBWI76_RS06030 and BWI76_RS06035 are separated by 89 nucleotides BWI76_RS06025: BWI76_RS06025 - amino acid transporter, at 1,264,437 to 1,265,843 _RS06025 BWI76_RS06030: BWI76_RS06030 - homocysteine S-methyltransferase, at 1,265,830 to 1,266,762 _RS06030 BWI76_RS06035: BWI76_RS06035 - ferric transporter ATP-binding subunit, at 1,266,852 to 1,267,898 _RS06035 Position (kb) 1265 1266 1267Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1264.883 kb on + strand, within BWI76_RS06025at 1264.920 kb on + strand, within BWI76_RS06025at 1264.952 kb on - strand, within BWI76_RS06025at 1265.350 kb on + strand, within BWI76_RS06025at 1265.492 kb on + strand, within BWI76_RS06025at 1265.492 kb on + strand, within BWI76_RS06025at 1265.648 kb on - strand, within BWI76_RS06025at 1265.727 kb on - strandat 1265.774 kb on + strandat 1265.929 kb on - strand, within BWI76_RS06030at 1265.977 kb on - strand, within BWI76_RS06030at 1266.125 kb on + strand, within BWI76_RS06030at 1266.148 kb on - strand, within BWI76_RS06030at 1266.405 kb on + strand, within BWI76_RS06030at 1266.410 kb on + strand, within BWI76_RS06030at 1266.410 kb on + strand, within BWI76_RS06030at 1266.410 kb on + strand, within BWI76_RS06030at 1266.418 kb on - strand, within BWI76_RS06030at 1266.418 kb on - strand, within BWI76_RS06030at 1266.764 kb on - strandat 1266.764 kb on - strandat 1266.786 kb on + strandat 1266.804 kb on - strandat 1266.831 kb on - strandat 1267.007 kb on - strand, within BWI76_RS06035at 1267.073 kb on - strand, within BWI76_RS06035at 1267.399 kb on + strand, within BWI76_RS06035at 1267.506 kb on - strand, within BWI76_RS06035

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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1,264,883 + BWI76_RS06025 0.32 +0.3
1,264,920 + BWI76_RS06025 0.34 -0.7
1,264,952 - BWI76_RS06025 0.37 +0.1
1,265,350 + BWI76_RS06025 0.65 -0.4
1,265,492 + BWI76_RS06025 0.75 -0.3
1,265,492 + BWI76_RS06025 0.75 +0.2
1,265,648 - BWI76_RS06025 0.86 +0.7
1,265,727 - -0.0
1,265,774 + -1.1
1,265,929 - BWI76_RS06030 0.11 -0.9
1,265,977 - BWI76_RS06030 0.16 -2.8
1,266,125 + BWI76_RS06030 0.32 -0.8
1,266,148 - BWI76_RS06030 0.34 -0.4
1,266,405 + BWI76_RS06030 0.62 +0.2
1,266,410 + BWI76_RS06030 0.62 -2.3
1,266,410 + BWI76_RS06030 0.62 -2.7
1,266,410 + BWI76_RS06030 0.62 -0.0
1,266,418 - BWI76_RS06030 0.63 -4.6
1,266,418 - BWI76_RS06030 0.63 -0.6
1,266,764 - -0.2
1,266,764 - -1.8
1,266,786 + -0.1
1,266,804 - +0.2
1,266,831 - +1.2
1,267,007 - BWI76_RS06035 0.15 +0.0
1,267,073 - BWI76_RS06035 0.21 +0.1
1,267,399 + BWI76_RS06035 0.52 +1.2
1,267,506 - BWI76_RS06035 0.62 +0.9

Or see this region's nucleotide sequence