Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04235 and BWI76_RS04240 are separated by 212 nucleotides BWI76_RS04240 and BWI76_RS04245 are separated by 81 nucleotides
BWI76_RS04235: BWI76_RS04235 - tRNA/rRNA methyltransferase, at 871,476 to 872,162
_RS04235
BWI76_RS04240: BWI76_RS04240 - thr operon leader peptide, at 872,375 to 872,443
_RS04240
BWI76_RS04245: BWI76_RS04245 - bifunctional aspartate kinase/homoserine dehydrogenase I, at 872,525 to 874,987
_RS04245
Position (kb) 872
873 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 871.620 kb on - strand, within BWI76_RS04235 at 871.624 kb on + strand, within BWI76_RS04235 at 871.632 kb on - strand, within BWI76_RS04235 at 871.784 kb on + strand, within BWI76_RS04235 at 871.868 kb on - strand, within BWI76_RS04235 at 871.870 kb on - strand, within BWI76_RS04235 at 871.886 kb on + strand, within BWI76_RS04235 at 871.903 kb on + strand, within BWI76_RS04235 at 871.903 kb on + strand, within BWI76_RS04235 at 871.911 kb on - strand, within BWI76_RS04235 at 871.930 kb on - strand, within BWI76_RS04235 at 872.053 kb on + strand, within BWI76_RS04235 at 872.061 kb on - strand, within BWI76_RS04235 at 872.061 kb on - strand, within BWI76_RS04235 at 872.071 kb on + strand, within BWI76_RS04235 at 872.079 kb on - strand, within BWI76_RS04235 at 872.110 kb on + strand at 872.110 kb on + strand at 872.118 kb on + strand at 872.123 kb on - strand at 872.337 kb on + strand at 872.362 kb on + strand at 872.368 kb on - strand at 872.433 kb on - strand, within BWI76_RS04240 at 872.433 kb on - strand, within BWI76_RS04240 at 872.473 kb on + strand at 872.473 kb on + strand at 872.473 kb on + strand at 872.475 kb on + strand at 872.475 kb on + strand at 872.481 kb on - strand at 872.515 kb on + strand at 872.612 kb on - strand at 872.625 kb on + strand at 872.754 kb on - strand at 872.844 kb on + strand, within BWI76_RS04245 at 872.844 kb on + strand, within BWI76_RS04245 at 872.844 kb on + strand, within BWI76_RS04245 at 872.844 kb on + strand, within BWI76_RS04245 at 872.852 kb on - strand, within BWI76_RS04245 at 872.852 kb on - strand, within BWI76_RS04245 at 872.852 kb on - strand, within BWI76_RS04245 at 872.852 kb on - strand, within BWI76_RS04245 at 872.852 kb on - strand, within BWI76_RS04245 at 872.882 kb on - strand, within BWI76_RS04245 at 872.935 kb on + strand, within BWI76_RS04245 at 873.226 kb on - strand, within BWI76_RS04245 at 873.292 kb on - strand, within BWI76_RS04245 at 873.295 kb on + strand, within BWI76_RS04245
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 871,620 - BWI76_RS04235 0.21 +0.3 871,624 + BWI76_RS04235 0.22 +0.0 871,632 - BWI76_RS04235 0.23 +0.3 871,784 + BWI76_RS04235 0.45 -1.7 871,868 - BWI76_RS04235 0.57 -2.8 871,870 - BWI76_RS04235 0.57 +1.0 871,886 + BWI76_RS04235 0.60 +0.7 871,903 + BWI76_RS04235 0.62 -0.9 871,903 + BWI76_RS04235 0.62 +0.5 871,911 - BWI76_RS04235 0.63 -0.2 871,930 - BWI76_RS04235 0.66 -3.1 872,053 + BWI76_RS04235 0.84 -1.1 872,061 - BWI76_RS04235 0.85 +1.0 872,061 - BWI76_RS04235 0.85 -0.0 872,071 + BWI76_RS04235 0.87 +0.4 872,079 - BWI76_RS04235 0.88 +2.3 872,110 + -1.2 872,110 + +0.1 872,118 + +3.9 872,123 - -0.2 872,337 + -0.1 872,362 + +0.1 872,368 - +1.3 872,433 - BWI76_RS04240 0.84 -1.2 872,433 - BWI76_RS04240 0.84 +1.3 872,473 + -1.9 872,473 + +0.2 872,473 + -1.6 872,475 + +2.7 872,475 + -1.0 872,481 - +1.7 872,515 + +0.8 872,612 - +0.6 872,625 + -1.8 872,754 - -0.5 872,844 + BWI76_RS04245 0.13 -2.0 872,844 + BWI76_RS04245 0.13 -0.3 872,844 + BWI76_RS04245 0.13 -3.0 872,844 + BWI76_RS04245 0.13 -1.8 872,852 - BWI76_RS04245 0.13 -2.7 872,852 - BWI76_RS04245 0.13 -1.5 872,852 - BWI76_RS04245 0.13 +0.1 872,852 - BWI76_RS04245 0.13 -0.7 872,852 - BWI76_RS04245 0.13 -4.2 872,882 - BWI76_RS04245 0.14 -0.6 872,935 + BWI76_RS04245 0.17 -1.6 873,226 - BWI76_RS04245 0.28 +0.3 873,292 - BWI76_RS04245 0.31 -1.2 873,295 + BWI76_RS04245 0.31 -1.7
Or see this region's nucleotide sequence