Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS03120

Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS03115 and BWI76_RS03120 are separated by 139 nucleotidesBWI76_RS03120 and BWI76_RS03125 are separated by 5 nucleotidesBWI76_RS03125 and BWI76_RS03130 are separated by 10 nucleotides BWI76_RS03115: BWI76_RS03115 - ABC transporter substrate-binding protein, at 641,872 to 643,182 _RS03115 BWI76_RS03120: BWI76_RS03120 - ABC transporter ATP-binding protein, at 643,322 to 644,392 _RS03120 BWI76_RS03125: BWI76_RS03125 - phosphodiesterase, at 644,398 to 645,222 _RS03125 BWI76_RS03130: BWI76_RS03130 - putative SN-glycerol-3-phosphate transport system permease, at 645,233 to 646,120 _RS03130 Position (kb) 643 644 645Strain fitness (log2 ratio) -2 -1 0 1 2 3at 642.365 kb on + strand, within BWI76_RS03115at 642.570 kb on - strand, within BWI76_RS03115at 642.648 kb on + strand, within BWI76_RS03115at 642.687 kb on - strand, within BWI76_RS03115at 642.773 kb on - strand, within BWI76_RS03115at 642.859 kb on - strand, within BWI76_RS03115at 642.867 kb on - strand, within BWI76_RS03115at 642.984 kb on + strand, within BWI76_RS03115at 643.062 kb on - strandat 643.079 kb on - strandat 643.137 kb on + strandat 643.137 kb on + strandat 643.137 kb on + strandat 643.137 kb on + strandat 643.137 kb on + strandat 643.145 kb on - strandat 643.145 kb on - strandat 643.145 kb on - strandat 643.145 kb on - strandat 643.145 kb on - strandat 643.149 kb on + strandat 643.237 kb on + strandat 643.237 kb on + strandat 643.245 kb on - strandat 643.458 kb on - strand, within BWI76_RS03120at 643.468 kb on - strand, within BWI76_RS03120at 643.562 kb on + strand, within BWI76_RS03120at 643.628 kb on + strand, within BWI76_RS03120at 643.736 kb on + strand, within BWI76_RS03120at 643.744 kb on - strand, within BWI76_RS03120at 643.827 kb on - strand, within BWI76_RS03120at 644.049 kb on + strand, within BWI76_RS03120at 644.247 kb on - strand, within BWI76_RS03120at 644.269 kb on - strand, within BWI76_RS03120at 644.616 kb on + strandat 644.699 kb on - strand, within BWI76_RS03125at 644.908 kb on - strand, within BWI76_RS03125at 644.965 kb on - strand, within BWI76_RS03125at 645.084 kb on + strand, within BWI76_RS03125at 645.103 kb on + strand, within BWI76_RS03125at 645.103 kb on + strand, within BWI76_RS03125at 645.105 kb on + strand, within BWI76_RS03125

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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642,365 + BWI76_RS03115 0.38 +0.4
642,570 - BWI76_RS03115 0.53 -0.3
642,648 + BWI76_RS03115 0.59 +0.5
642,687 - BWI76_RS03115 0.62 -0.1
642,773 - BWI76_RS03115 0.69 -0.5
642,859 - BWI76_RS03115 0.75 +0.8
642,867 - BWI76_RS03115 0.76 -1.6
642,984 + BWI76_RS03115 0.85 -0.7
643,062 - -0.0
643,079 - -0.9
643,137 + +1.2
643,137 + -1.7
643,137 + +1.3
643,137 + -1.7
643,137 + +0.9
643,145 - +0.5
643,145 - -0.6
643,145 - -0.1
643,145 - -1.6
643,145 - +0.8
643,149 + -0.6
643,237 + +0.3
643,237 + +0.4
643,245 - -1.9
643,458 - BWI76_RS03120 0.13 +0.3
643,468 - BWI76_RS03120 0.14 +1.1
643,562 + BWI76_RS03120 0.22 +0.7
643,628 + BWI76_RS03120 0.29 +0.6
643,736 + BWI76_RS03120 0.39 +1.7
643,744 - BWI76_RS03120 0.39 +0.1
643,827 - BWI76_RS03120 0.47 -0.3
644,049 + BWI76_RS03120 0.68 +0.8
644,247 - BWI76_RS03120 0.86 +1.0
644,269 - BWI76_RS03120 0.88 +0.6
644,616 + +1.4
644,699 - BWI76_RS03125 0.36 +0.1
644,908 - BWI76_RS03125 0.62 -1.8
644,965 - BWI76_RS03125 0.69 +0.2
645,084 + BWI76_RS03125 0.83 +0.3
645,103 + BWI76_RS03125 0.85 +2.8
645,103 + BWI76_RS03125 0.85 -1.7
645,105 + BWI76_RS03125 0.86 +0.6

Or see this region's nucleotide sequence