Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00845

Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00840 and BWI76_RS00845 are separated by 184 nucleotidesBWI76_RS00845 and BWI76_RS00850 are separated by 9 nucleotidesBWI76_RS00850 and BWI76_RS00855 are separated by 77 nucleotides BWI76_RS00840: BWI76_RS00840 - acetylornithine deacetylase, at 173,585 to 174,736 _RS00840 BWI76_RS00845: BWI76_RS00845 - N-acetyl-gamma-glutamyl-phosphate reductase, at 174,921 to 175,925 _RS00845 BWI76_RS00850: BWI76_RS00850 - acetylglutamate kinase, at 175,935 to 176,708 _RS00850 BWI76_RS00855: BWI76_RS00855 - argininosuccinate lyase, at 176,786 to 178,159 _RS00855 Position (kb) 174 175 176Strain fitness (log2 ratio) -2 -1 0 1at 173.960 kb on - strand, within BWI76_RS00840at 173.960 kb on - strand, within BWI76_RS00840at 174.257 kb on + strand, within BWI76_RS00840at 174.352 kb on + strand, within BWI76_RS00840at 174.432 kb on - strand, within BWI76_RS00840at 174.432 kb on - strand, within BWI76_RS00840at 174.445 kb on + strand, within BWI76_RS00840at 174.445 kb on + strand, within BWI76_RS00840at 174.453 kb on - strand, within BWI76_RS00840at 174.497 kb on - strand, within BWI76_RS00840at 174.674 kb on + strandat 174.707 kb on - strandat 174.966 kb on + strandat 174.996 kb on + strandat 175.207 kb on + strand, within BWI76_RS00845at 175.291 kb on + strand, within BWI76_RS00845at 175.291 kb on + strand, within BWI76_RS00845at 175.329 kb on - strand, within BWI76_RS00845at 175.483 kb on - strand, within BWI76_RS00845at 175.498 kb on - strand, within BWI76_RS00845at 175.593 kb on + strand, within BWI76_RS00845at 175.709 kb on - strand, within BWI76_RS00845at 175.714 kb on - strand, within BWI76_RS00845at 175.739 kb on + strand, within BWI76_RS00845at 175.775 kb on - strand, within BWI76_RS00845at 175.803 kb on + strand, within BWI76_RS00845at 175.825 kb on - strandat 176.129 kb on + strand, within BWI76_RS00850at 176.171 kb on + strand, within BWI76_RS00850at 176.235 kb on - strand, within BWI76_RS00850at 176.263 kb on + strand, within BWI76_RS00850at 176.350 kb on + strand, within BWI76_RS00850at 176.350 kb on + strand, within BWI76_RS00850at 176.358 kb on - strand, within BWI76_RS00850at 176.583 kb on - strand, within BWI76_RS00850at 176.612 kb on - strand, within BWI76_RS00850at 176.652 kb on + strandat 176.655 kb on - strandat 176.669 kb on + strandat 176.669 kb on + strandat 176.737 kb on + strandat 176.861 kb on + strandat 176.876 kb on + strandat 176.917 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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173,960 - BWI76_RS00840 0.33 +0.4
173,960 - BWI76_RS00840 0.33 -1.6
174,257 + BWI76_RS00840 0.58 -0.5
174,352 + BWI76_RS00840 0.67 +0.4
174,432 - BWI76_RS00840 0.74 +1.1
174,432 - BWI76_RS00840 0.74 +0.2
174,445 + BWI76_RS00840 0.75 -0.4
174,445 + BWI76_RS00840 0.75 +0.1
174,453 - BWI76_RS00840 0.75 -0.5
174,497 - BWI76_RS00840 0.79 +0.4
174,674 + +0.1
174,707 - +1.2
174,966 + -0.6
174,996 + -0.1
175,207 + BWI76_RS00845 0.28 -2.4
175,291 + BWI76_RS00845 0.37 -1.2
175,291 + BWI76_RS00845 0.37 +1.4
175,329 - BWI76_RS00845 0.41 -2.4
175,483 - BWI76_RS00845 0.56 -0.4
175,498 - BWI76_RS00845 0.57 -1.7
175,593 + BWI76_RS00845 0.67 -1.7
175,709 - BWI76_RS00845 0.78 -1.6
175,714 - BWI76_RS00845 0.79 -1.0
175,739 + BWI76_RS00845 0.81 -1.6
175,775 - BWI76_RS00845 0.85 -1.7
175,803 + BWI76_RS00845 0.88 -1.3
175,825 - -1.5
176,129 + BWI76_RS00850 0.25 -1.1
176,171 + BWI76_RS00850 0.30 -1.7
176,235 - BWI76_RS00850 0.39 -0.7
176,263 + BWI76_RS00850 0.42 -0.4
176,350 + BWI76_RS00850 0.54 -2.5
176,350 + BWI76_RS00850 0.54 -0.8
176,358 - BWI76_RS00850 0.55 -0.4
176,583 - BWI76_RS00850 0.84 -1.2
176,612 - BWI76_RS00850 0.87 -0.4
176,652 + -0.8
176,655 - -1.7
176,669 + -1.7
176,669 + -0.4
176,737 + +0.6
176,861 + -2.3
176,876 + -1.1
176,917 + -0.0

Or see this region's nucleotide sequence