Experiment: MoYLS4 with Ammonium Persulfate 2.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysW and cysA overlap by 4 nucleotides cysA and acs are separated by 85 nucleotides acs and DVU0749 are separated by 49 nucleotides
DVU0746: cysW - ABC transporter, permease protein (TIGR), at 835,639 to 836,385
cysW
DVU0747: cysA - ABC transporter, ATP-binding protein (TIGR), at 836,382 to 837,020
cysA
DVU0748: acs - acetyl-CoA synthetase (TIGR), at 837,106 to 839,052
acs
DVU0749: DVU0749 - DNA-binding response regulator (TIGR), at 839,102 to 840,031
DVU0749
Position (kb) 837
838
839
840 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 836.143 kb on + strand, within cysW at 836.372 kb on + strand at 836.709 kb on + strand, within cysA at 836.751 kb on - strand, within cysA at 836.838 kb on + strand, within cysA at 836.843 kb on + strand, within cysA at 836.871 kb on + strand, within cysA at 836.953 kb on + strand, within cysA at 837.048 kb on - strand at 837.118 kb on - strand at 837.136 kb on - strand at 837.145 kb on + strand at 837.228 kb on - strand at 837.268 kb on - strand at 837.276 kb on + strand at 837.287 kb on + strand at 837.430 kb on - strand, within acs at 837.787 kb on + strand, within acs at 837.810 kb on + strand, within acs at 837.818 kb on - strand, within acs at 837.826 kb on + strand, within acs at 837.905 kb on + strand, within acs at 837.905 kb on + strand, within acs at 837.907 kb on + strand, within acs at 837.960 kb on + strand, within acs at 837.961 kb on - strand, within acs at 838.066 kb on + strand, within acs at 838.086 kb on - strand, within acs at 838.101 kb on + strand, within acs at 838.109 kb on - strand, within acs at 838.162 kb on + strand, within acs at 838.202 kb on + strand, within acs at 838.210 kb on - strand, within acs at 838.370 kb on + strand, within acs at 838.430 kb on + strand, within acs at 838.485 kb on + strand, within acs at 838.485 kb on + strand, within acs at 838.547 kb on + strand, within acs at 838.593 kb on - strand, within acs at 838.618 kb on + strand, within acs at 838.714 kb on + strand, within acs at 838.807 kb on - strand, within acs at 838.930 kb on - strand at 838.953 kb on + strand at 838.984 kb on + strand at 839.163 kb on - strand at 839.163 kb on - strand at 839.396 kb on - strand, within DVU0749 at 839.465 kb on - strand, within DVU0749 at 839.469 kb on + strand, within DVU0749 at 839.469 kb on + strand, within DVU0749 at 839.469 kb on + strand, within DVU0749 at 839.487 kb on - strand, within DVU0749 at 839.487 kb on - strand, within DVU0749 at 839.521 kb on + strand, within DVU0749 at 839.543 kb on + strand, within DVU0749 at 839.543 kb on + strand, within DVU0749 at 839.680 kb on + strand, within DVU0749 at 839.681 kb on + strand, within DVU0749 at 839.861 kb on - strand, within DVU0749 at 839.885 kb on + strand, within DVU0749 at 839.885 kb on + strand, within DVU0749 at 839.905 kb on - strand, within DVU0749 at 839.912 kb on - strand, within DVU0749 at 839.932 kb on + strand, within DVU0749 at 839.989 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Ammonium Persulfate 2.5mM remove 836,143 + cysW DVU0746 0.67 -1.0 836,372 + +0.1 836,709 + cysA DVU0747 0.51 +0.5 836,751 - cysA DVU0747 0.58 -0.5 836,838 + cysA DVU0747 0.71 -0.6 836,843 + cysA DVU0747 0.72 +0.2 836,871 + cysA DVU0747 0.77 -0.7 836,953 + cysA DVU0747 0.89 -0.7 837,048 - -1.8 837,118 - -0.8 837,136 - -0.2 837,145 + -0.5 837,228 - -0.2 837,268 - -0.5 837,276 + -0.3 837,287 + +0.4 837,430 - acs DVU0748 0.17 -0.1 837,787 + acs DVU0748 0.35 +1.2 837,810 + acs DVU0748 0.36 -2.2 837,818 - acs DVU0748 0.37 +0.7 837,826 + acs DVU0748 0.37 -0.8 837,905 + acs DVU0748 0.41 +0.2 837,905 + acs DVU0748 0.41 -0.9 837,907 + acs DVU0748 0.41 -0.6 837,960 + acs DVU0748 0.44 -0.4 837,961 - acs DVU0748 0.44 -0.9 838,066 + acs DVU0748 0.49 -2.0 838,086 - acs DVU0748 0.50 -2.9 838,101 + acs DVU0748 0.51 -0.8 838,109 - acs DVU0748 0.52 -0.8 838,162 + acs DVU0748 0.54 -0.6 838,202 + acs DVU0748 0.56 -1.3 838,210 - acs DVU0748 0.57 -0.8 838,370 + acs DVU0748 0.65 -0.9 838,430 + acs DVU0748 0.68 +0.4 838,485 + acs DVU0748 0.71 +1.7 838,485 + acs DVU0748 0.71 -0.5 838,547 + acs DVU0748 0.74 +0.1 838,593 - acs DVU0748 0.76 -1.7 838,618 + acs DVU0748 0.78 -1.1 838,714 + acs DVU0748 0.83 -0.7 838,807 - acs DVU0748 0.87 -0.1 838,930 - +0.2 838,953 + -0.8 838,984 + -1.2 839,163 - -0.5 839,163 - -0.6 839,396 - DVU0749 0.32 -0.6 839,465 - DVU0749 0.39 -1.3 839,469 + DVU0749 0.39 +1.0 839,469 + DVU0749 0.39 +0.2 839,469 + DVU0749 0.39 +0.4 839,487 - DVU0749 0.41 +1.1 839,487 - DVU0749 0.41 -0.2 839,521 + DVU0749 0.45 +0.7 839,543 + DVU0749 0.47 +0.1 839,543 + DVU0749 0.47 +0.2 839,680 + DVU0749 0.62 -0.5 839,681 + DVU0749 0.62 -0.2 839,861 - DVU0749 0.82 -1.1 839,885 + DVU0749 0.84 -0.2 839,885 + DVU0749 0.84 -1.5 839,905 - DVU0749 0.86 +0.3 839,912 - DVU0749 0.87 -0.2 839,932 + DVU0749 0.89 -0.6 839,989 + +0.1
Or see this region's nucleotide sequence