Experiment: Lactitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1480 and SMa1483 are separated by 235 nucleotides SMa1483 and SMa5009 are separated by 60 nucleotides SMa5009 and SMa1485 overlap by 4 nucleotides
SMa1480: SMa1480 - LysR family transcriptional regulator, at 814,015 to 814,902
SMa1480
SMa1483: SMa1483 - dehydrogenase, at 815,138 to 817,558
SMa1483
SMa5009: SMa5009 - hypothetical protein, at 817,619 to 817,969
SMa5009
SMa1485: SMa1485 - Permease, MFS, at 817,966 to 819,255
SMa1485
Position (kb) 815
816
817
818 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 814.150 kb on + strand, within SMa1480 at 814.151 kb on - strand, within SMa1480 at 814.261 kb on - strand, within SMa1480 at 814.386 kb on - strand, within SMa1480 at 814.511 kb on + strand, within SMa1480 at 814.512 kb on - strand, within SMa1480 at 814.512 kb on - strand, within SMa1480 at 814.637 kb on + strand, within SMa1480 at 814.637 kb on + strand, within SMa1480 at 814.637 kb on + strand, within SMa1480 at 814.638 kb on - strand, within SMa1480 at 814.638 kb on - strand, within SMa1480 at 814.750 kb on + strand, within SMa1480 at 814.750 kb on + strand, within SMa1480 at 814.750 kb on + strand, within SMa1480 at 814.751 kb on - strand, within SMa1480 at 814.751 kb on - strand, within SMa1480 at 814.751 kb on - strand, within SMa1480 at 814.751 kb on - strand, within SMa1480 at 814.810 kb on + strand, within SMa1480 at 814.811 kb on - strand, within SMa1480 at 814.811 kb on - strand, within SMa1480 at 814.814 kb on - strand at 815.136 kb on - strand at 815.199 kb on - strand at 815.335 kb on + strand at 815.335 kb on + strand at 815.336 kb on - strand at 815.336 kb on - strand at 815.336 kb on - strand at 815.336 kb on - strand at 815.336 kb on - strand at 815.348 kb on + strand at 815.349 kb on - strand at 815.408 kb on + strand, within SMa1483 at 815.558 kb on + strand, within SMa1483 at 815.559 kb on - strand, within SMa1483 at 815.559 kb on - strand, within SMa1483 at 815.606 kb on + strand, within SMa1483 at 815.606 kb on + strand, within SMa1483 at 815.640 kb on + strand, within SMa1483 at 815.641 kb on - strand, within SMa1483 at 815.718 kb on + strand, within SMa1483 at 815.718 kb on + strand, within SMa1483 at 815.718 kb on + strand, within SMa1483 at 815.718 kb on + strand, within SMa1483 at 815.718 kb on + strand, within SMa1483 at 815.719 kb on - strand, within SMa1483 at 815.719 kb on - strand, within SMa1483 at 815.719 kb on - strand, within SMa1483 at 815.817 kb on - strand, within SMa1483 at 815.895 kb on - strand, within SMa1483 at 815.895 kb on - strand at 815.898 kb on - strand, within SMa1483 at 815.937 kb on - strand, within SMa1483 at 816.118 kb on + strand, within SMa1483 at 816.159 kb on - strand, within SMa1483 at 816.159 kb on - strand, within SMa1483 at 816.159 kb on - strand, within SMa1483 at 816.332 kb on + strand, within SMa1483 at 816.333 kb on - strand, within SMa1483 at 816.605 kb on + strand, within SMa1483 at 816.668 kb on - strand, within SMa1483 at 816.794 kb on + strand, within SMa1483 at 816.794 kb on + strand, within SMa1483 at 817.187 kb on + strand, within SMa1483 at 817.188 kb on - strand, within SMa1483 at 817.404 kb on - strand at 817.406 kb on - strand at 817.597 kb on - strand at 817.731 kb on + strand at 817.731 kb on + strand, within SMa5009 at 817.732 kb on - strand, within SMa5009 at 817.761 kb on + strand, within SMa5009 at 817.762 kb on - strand, within SMa5009 at 817.762 kb on - strand, within SMa5009 at 817.762 kb on - strand, within SMa5009 at 817.764 kb on + strand, within SMa5009 at 817.764 kb on + strand, within SMa5009 at 817.764 kb on + strand, within SMa5009 at 817.764 kb on + strand, within SMa5009 at 817.764 kb on + strand, within SMa5009 at 817.764 kb on + strand, within SMa5009 at 817.764 kb on + strand, within SMa5009 at 817.765 kb on - strand, within SMa5009 at 817.834 kb on - strand, within SMa5009 at 817.858 kb on - strand, within SMa5009 at 817.858 kb on - strand, within SMa5009 at 817.973 kb on - strand at 818.420 kb on + strand, within SMa1485 at 818.420 kb on + strand, within SMa1485
Per-strain Table
Position Strand Gene LocusTag Fraction Lactitol (C) remove 814,150 + SMa1480 0.15 -0.2 814,151 - SMa1480 0.15 +0.3 814,261 - SMa1480 0.28 +0.0 814,386 - SMa1480 0.42 -0.4 814,511 + SMa1480 0.56 +0.4 814,512 - SMa1480 0.56 +0.4 814,512 - SMa1480 0.56 +1.7 814,637 + SMa1480 0.70 -1.6 814,637 + SMa1480 0.70 +0.7 814,637 + SMa1480 0.70 -1.0 814,638 - SMa1480 0.70 +0.2 814,638 - SMa1480 0.70 +1.0 814,750 + SMa1480 0.83 -0.1 814,750 + SMa1480 0.83 +0.3 814,750 + SMa1480 0.83 +0.1 814,751 - SMa1480 0.83 -1.1 814,751 - SMa1480 0.83 +1.3 814,751 - SMa1480 0.83 -0.4 814,751 - SMa1480 0.83 -0.3 814,810 + SMa1480 0.90 -1.9 814,811 - SMa1480 0.90 -0.5 814,811 - SMa1480 0.90 +1.6 814,814 - -0.9 815,136 - -0.3 815,199 - +0.2 815,335 + -0.8 815,335 + -1.7 815,336 - +0.1 815,336 - -1.0 815,336 - +0.6 815,336 - -0.5 815,336 - +1.1 815,348 + -0.5 815,349 - +0.3 815,408 + SMa1483 0.11 -1.6 815,558 + SMa1483 0.17 -0.7 815,559 - SMa1483 0.17 -0.6 815,559 - SMa1483 0.17 +0.1 815,606 + SMa1483 0.19 -1.3 815,606 + SMa1483 0.19 +1.0 815,640 + SMa1483 0.21 -0.2 815,641 - SMa1483 0.21 +0.1 815,718 + SMa1483 0.24 -0.1 815,718 + SMa1483 0.24 -0.4 815,718 + SMa1483 0.24 -0.2 815,718 + SMa1483 0.24 -0.3 815,718 + SMa1483 0.24 -0.2 815,719 - SMa1483 0.24 +0.1 815,719 - SMa1483 0.24 -1.0 815,719 - SMa1483 0.24 +0.6 815,817 - SMa1483 0.28 -1.4 815,895 - SMa1483 0.31 -2.9 815,895 - +0.7 815,898 - SMa1483 0.31 -0.7 815,937 - SMa1483 0.33 -0.6 816,118 + SMa1483 0.40 +0.2 816,159 - SMa1483 0.42 -0.2 816,159 - SMa1483 0.42 +0.3 816,159 - SMa1483 0.42 +0.3 816,332 + SMa1483 0.49 +3.0 816,333 - SMa1483 0.49 -0.4 816,605 + SMa1483 0.61 +0.2 816,668 - SMa1483 0.63 -0.9 816,794 + SMa1483 0.68 +0.1 816,794 + SMa1483 0.68 -1.0 817,187 + SMa1483 0.85 -0.2 817,188 - SMa1483 0.85 -0.2 817,404 - +0.2 817,406 - +0.2 817,597 - +0.2 817,731 + -0.3 817,731 + SMa5009 0.32 +0.3 817,732 - SMa5009 0.32 -0.3 817,761 + SMa5009 0.40 -0.5 817,762 - SMa5009 0.41 -0.3 817,762 - SMa5009 0.41 -0.8 817,762 - SMa5009 0.41 -1.2 817,764 + SMa5009 0.41 -0.3 817,764 + SMa5009 0.41 -0.9 817,764 + SMa5009 0.41 -0.1 817,764 + SMa5009 0.41 +2.3 817,764 + SMa5009 0.41 -0.7 817,764 + SMa5009 0.41 +0.1 817,764 + SMa5009 0.41 -0.1 817,765 - SMa5009 0.42 -1.0 817,834 - SMa5009 0.61 +0.9 817,858 - SMa5009 0.68 -0.2 817,858 - SMa5009 0.68 -0.5 817,973 - -0.1 818,420 + SMa1485 0.35 +0.0 818,420 + SMa1485 0.35 +1.3
Or see this region's nucleotide sequence