Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25060 and BWI76_RS25065 are separated by 107 nucleotides BWI76_RS25065 and BWI76_RS25070 overlap by 42 nucleotides BWI76_RS25070 and BWI76_RS25075 are separated by 71 nucleotides BWI76_RS25075 and BWI76_RS25080 are separated by 147 nucleotides
BWI76_RS25060: BWI76_RS25060 - lipoprotein NlpI, at 5,217,454 to 5,218,338
_RS25060
BWI76_RS25065: BWI76_RS25065 - polyribonucleotide nucleotidyltransferase, at 5,218,446 to 5,220,584
_RS25065
BWI76_RS25070: BWI76_RS25070 - hypothetical protein, at 5,220,543 to 5,220,754
_RS25070
BWI76_RS25075: BWI76_RS25075 - 30S ribosomal protein S15, at 5,220,826 to 5,221,095
_RS25075
BWI76_RS25080: BWI76_RS25080 - tRNA pseudouridine(55) synthase, at 5,221,243 to 5,222,187
_RS25080
Position (kb) 5218
5219
5220
5221 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5217.685 kb on - strand, within BWI76_RS25060 at 5217.919 kb on - strand, within BWI76_RS25060 at 5218.150 kb on - strand, within BWI76_RS25060 at 5218.177 kb on - strand, within BWI76_RS25060 at 5218.197 kb on - strand, within BWI76_RS25060 at 5218.294 kb on + strand at 5219.314 kb on + strand, within BWI76_RS25065 at 5219.551 kb on + strand, within BWI76_RS25065 at 5219.825 kb on + strand, within BWI76_RS25065 at 5220.072 kb on - strand, within BWI76_RS25065 at 5220.091 kb on + strand, within BWI76_RS25065 at 5220.430 kb on + strand at 5220.472 kb on + strand at 5220.681 kb on - strand, within BWI76_RS25070 at 5220.709 kb on - strand at 5221.250 kb on + strand at 5221.407 kb on - strand, within BWI76_RS25080 at 5221.453 kb on + strand, within BWI76_RS25080 at 5221.520 kb on + strand, within BWI76_RS25080
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 5,217,685 - BWI76_RS25060 0.26 +0.7 5,217,919 - BWI76_RS25060 0.53 -3.0 5,218,150 - BWI76_RS25060 0.79 -0.7 5,218,177 - BWI76_RS25060 0.82 -1.6 5,218,197 - BWI76_RS25060 0.84 -0.7 5,218,294 + -0.4 5,219,314 + BWI76_RS25065 0.41 +0.1 5,219,551 + BWI76_RS25065 0.52 +1.1 5,219,825 + BWI76_RS25065 0.64 -1.7 5,220,072 - BWI76_RS25065 0.76 -1.2 5,220,091 + BWI76_RS25065 0.77 -2.2 5,220,430 + -1.2 5,220,472 + +1.1 5,220,681 - BWI76_RS25070 0.65 -1.5 5,220,709 - +1.1 5,221,250 + +0.5 5,221,407 - BWI76_RS25080 0.17 +0.6 5,221,453 + BWI76_RS25080 0.22 +1.1 5,221,520 + BWI76_RS25080 0.29 +0.4
Or see this region's nucleotide sequence