Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23030

Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23015 and BWI76_RS23020 are separated by 8 nucleotidesBWI76_RS23020 and BWI76_RS23025 overlap by 14 nucleotidesBWI76_RS23025 and BWI76_RS23030 are separated by 1 nucleotidesBWI76_RS23030 and BWI76_RS23035 overlap by 4 nucleotidesBWI76_RS23035 and BWI76_RS23040 overlap by 4 nucleotides BWI76_RS23015: BWI76_RS23015 - cobalt transporter ATP-binding subunit, at 4,802,035 to 4,802,850 _RS23015 BWI76_RS23020: BWI76_RS23020 - cobalamin biosynthesis protein CbiQ, at 4,802,859 to 4,803,536 _RS23020 BWI76_RS23025: BWI76_RS23025 - cobalt ABC transporter substrate-binding protein CbiN, at 4,803,523 to 4,803,804 _RS23025 BWI76_RS23030: BWI76_RS23030 - cobalamin biosynthesis protein CbiM, at 4,803,806 to 4,804,543 _RS23030 BWI76_RS23035: BWI76_RS23035 - precorrin-2 C(20)-methyltransferase, at 4,804,540 to 4,805,253 _RS23035 BWI76_RS23040: BWI76_RS23040 - sirohydrochlorin cobaltochelatase, at 4,805,250 to 4,806,044 _RS23040 Position (kb) 4803 4804 4805Strain fitness (log2 ratio) -3 -2 -1 0 1at 4802.858 kb on - strandat 4802.875 kb on + strandat 4802.875 kb on + strandat 4803.106 kb on + strand, within BWI76_RS23020at 4803.114 kb on - strand, within BWI76_RS23020at 4803.392 kb on - strand, within BWI76_RS23020at 4803.606 kb on - strand, within BWI76_RS23025at 4803.768 kb on - strand, within BWI76_RS23025at 4804.086 kb on - strand, within BWI76_RS23030at 4804.305 kb on + strand, within BWI76_RS23030at 4804.429 kb on + strandat 4804.681 kb on - strand, within BWI76_RS23035at 4804.823 kb on + strand, within BWI76_RS23035at 4804.904 kb on - strand, within BWI76_RS23035at 4804.994 kb on + strand, within BWI76_RS23035at 4805.015 kb on + strand, within BWI76_RS23035at 4805.376 kb on - strand, within BWI76_RS23040at 4805.517 kb on + strand, within BWI76_RS23040

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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4,802,858 - +0.8
4,802,875 + -0.1
4,802,875 + -0.7
4,803,106 + BWI76_RS23020 0.36 +0.9
4,803,114 - BWI76_RS23020 0.38 -1.1
4,803,392 - BWI76_RS23020 0.79 +1.0
4,803,606 - BWI76_RS23025 0.29 +0.9
4,803,768 - BWI76_RS23025 0.87 +0.7
4,804,086 - BWI76_RS23030 0.38 +1.2
4,804,305 + BWI76_RS23030 0.68 -0.7
4,804,429 + -0.9
4,804,681 - BWI76_RS23035 0.20 -0.8
4,804,823 + BWI76_RS23035 0.40 +0.1
4,804,904 - BWI76_RS23035 0.51 +0.4
4,804,994 + BWI76_RS23035 0.64 -2.8
4,805,015 + BWI76_RS23035 0.67 +0.3
4,805,376 - BWI76_RS23040 0.16 +0.9
4,805,517 + BWI76_RS23040 0.34 -0.4

Or see this region's nucleotide sequence