Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20605

Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20600 and BWI76_RS20605 are separated by 60 nucleotidesBWI76_RS20605 and BWI76_RS20610 are separated by 122 nucleotides BWI76_RS20600: BWI76_RS20600 - nucleoside permease, at 4,310,467 to 4,311,723 _RS20600 BWI76_RS20605: BWI76_RS20605 - purine-nucleoside phosphorylase, at 4,311,784 to 4,312,617 _RS20605 BWI76_RS20610: BWI76_RS20610 - LysR family transcriptional regulator, at 4,312,740 to 4,313,666 _RS20610 Position (kb) 4311 4312 4313Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4310.796 kb on + strand, within BWI76_RS20600at 4310.804 kb on - strand, within BWI76_RS20600at 4310.811 kb on - strand, within BWI76_RS20600at 4310.842 kb on + strand, within BWI76_RS20600at 4310.849 kb on + strand, within BWI76_RS20600at 4311.241 kb on - strand, within BWI76_RS20600at 4311.241 kb on - strand, within BWI76_RS20600at 4311.255 kb on + strand, within BWI76_RS20600at 4311.263 kb on - strand, within BWI76_RS20600at 4311.371 kb on - strand, within BWI76_RS20600at 4311.498 kb on - strand, within BWI76_RS20600at 4311.500 kb on - strand, within BWI76_RS20600at 4311.512 kb on - strand, within BWI76_RS20600at 4311.514 kb on - strand, within BWI76_RS20600at 4311.524 kb on - strand, within BWI76_RS20600at 4311.640 kb on + strandat 4311.811 kb on + strandat 4311.927 kb on + strand, within BWI76_RS20605at 4312.190 kb on - strand, within BWI76_RS20605at 4312.320 kb on + strand, within BWI76_RS20605at 4312.378 kb on + strand, within BWI76_RS20605at 4312.577 kb on + strandat 4312.621 kb on - strandat 4313.394 kb on - strand, within BWI76_RS20610at 4313.416 kb on + strand, within BWI76_RS20610at 4313.476 kb on + strand, within BWI76_RS20610at 4313.493 kb on - strand, within BWI76_RS20610at 4313.530 kb on - strand, within BWI76_RS20610at 4313.582 kb on + strandat 4313.590 kb on - strandat 4313.602 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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4,310,796 + BWI76_RS20600 0.26 -0.1
4,310,804 - BWI76_RS20600 0.27 +0.1
4,310,811 - BWI76_RS20600 0.27 +0.4
4,310,842 + BWI76_RS20600 0.30 +0.6
4,310,849 + BWI76_RS20600 0.30 -0.5
4,311,241 - BWI76_RS20600 0.62 -0.1
4,311,241 - BWI76_RS20600 0.62 +0.3
4,311,255 + BWI76_RS20600 0.63 +0.5
4,311,263 - BWI76_RS20600 0.63 -1.9
4,311,371 - BWI76_RS20600 0.72 +0.7
4,311,498 - BWI76_RS20600 0.82 -0.5
4,311,500 - BWI76_RS20600 0.82 +0.3
4,311,512 - BWI76_RS20600 0.83 -0.1
4,311,514 - BWI76_RS20600 0.83 +0.4
4,311,524 - BWI76_RS20600 0.84 -1.3
4,311,640 + +0.2
4,311,811 + -1.6
4,311,927 + BWI76_RS20605 0.17 +0.3
4,312,190 - BWI76_RS20605 0.49 +0.7
4,312,320 + BWI76_RS20605 0.64 +1.8
4,312,378 + BWI76_RS20605 0.71 +0.3
4,312,577 + -0.1
4,312,621 - +2.9
4,313,394 - BWI76_RS20610 0.71 +0.4
4,313,416 + BWI76_RS20610 0.73 +0.2
4,313,476 + BWI76_RS20610 0.79 -1.1
4,313,493 - BWI76_RS20610 0.81 +0.8
4,313,530 - BWI76_RS20610 0.85 +0.3
4,313,582 + -0.3
4,313,590 - -0.3
4,313,602 - +0.3

Or see this region's nucleotide sequence