Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20220 and BWI76_RS20225 are separated by 11 nucleotides BWI76_RS20225 and BWI76_RS20230 are separated by 86 nucleotides BWI76_RS20230 and BWI76_RS20235 are separated by 336 nucleotides
BWI76_RS20220: BWI76_RS20220 - sugar phosphatase, at 4,238,333 to 4,238,992
_RS20220
BWI76_RS20225: BWI76_RS20225 - hypothetical protein, at 4,239,004 to 4,239,498
_RS20225
BWI76_RS20230: BWI76_RS20230 - hypothetical protein, at 4,239,585 to 4,240,040
_RS20230
BWI76_RS20235: BWI76_RS20235 - acetate kinase, at 4,240,377 to 4,241,579
_RS20235
Position (kb) 4239
4240
4241 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4238.720 kb on - strand, within BWI76_RS20220 at 4238.738 kb on - strand, within BWI76_RS20220 at 4238.979 kb on + strand at 4238.979 kb on + strand at 4238.979 kb on + strand at 4238.979 kb on + strand at 4238.987 kb on - strand at 4239.045 kb on - strand at 4239.045 kb on - strand at 4239.226 kb on + strand, within BWI76_RS20225 at 4239.369 kb on + strand, within BWI76_RS20225 at 4239.469 kb on + strand at 4239.516 kb on + strand at 4239.637 kb on - strand, within BWI76_RS20230 at 4239.643 kb on + strand, within BWI76_RS20230 at 4240.035 kb on + strand at 4240.164 kb on - strand at 4240.747 kb on + strand, within BWI76_RS20235
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 4,238,720 - BWI76_RS20220 0.59 +0.3 4,238,738 - BWI76_RS20220 0.61 +0.2 4,238,979 + +0.4 4,238,979 + -0.4 4,238,979 + +2.4 4,238,979 + -1.4 4,238,987 - +0.2 4,239,045 - -0.1 4,239,045 - +0.7 4,239,226 + BWI76_RS20225 0.45 +0.2 4,239,369 + BWI76_RS20225 0.74 +0.5 4,239,469 + +0.3 4,239,516 + -2.7 4,239,637 - BWI76_RS20230 0.11 +1.6 4,239,643 + BWI76_RS20230 0.13 +0.9 4,240,035 + -1.4 4,240,164 - +1.1 4,240,747 + BWI76_RS20235 0.31 -2.3
Or see this region's nucleotide sequence