Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19925 and BWI76_RS19930 are separated by 185 nucleotides BWI76_RS19930 and BWI76_RS19935 are separated by 43 nucleotides BWI76_RS19935 and BWI76_RS19940 are separated by 112 nucleotides
BWI76_RS19925: BWI76_RS19925 - DNA gyrase subunit A, at 4,171,294 to 4,173,927
_RS19925
BWI76_RS19930: BWI76_RS19930 - bifunctional 3-demethylubiquinone 3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol methylase, at 4,174,113 to 4,174,841
_RS19930
BWI76_RS19935: BWI76_RS19935 - hypothetical protein, at 4,174,885 to 4,175,073
_RS19935
BWI76_RS19940: BWI76_RS19940 - ribonucleoside-diphosphate reductase subunit alpha, at 4,175,186 to 4,177,471
_RS19940
Position (kb) 4174
4175
4176 Strain fitness (log2 ratio) -1
0
1 at 4173.981 kb on + strand at 4174.048 kb on - strand at 4174.814 kb on + strand at 4174.881 kb on - strand at 4174.881 kb on - strand at 4174.959 kb on + strand, within BWI76_RS19935
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 4,173,981 + -0.6 4,174,048 - +0.9 4,174,814 + +0.1 4,174,881 - +0.8 4,174,881 - -0.3 4,174,959 + BWI76_RS19935 0.39 +0.9
Or see this region's nucleotide sequence