Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS19540

Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS19530 and BWI76_RS19535 are separated by 155 nucleotidesBWI76_RS19535 and BWI76_RS19540 are separated by 105 nucleotidesBWI76_RS19540 and BWI76_RS19545 are separated by 119 nucleotidesBWI76_RS19545 and BWI76_RS19550 are separated by 96 nucleotides BWI76_RS19530: BWI76_RS19530 - YIP1 family protein, at 4,082,420 to 4,083,007 _RS19530 BWI76_RS19535: BWI76_RS19535 - putative integral membrane protein, at 4,083,163 to 4,083,732 _RS19535 BWI76_RS19540: BWI76_RS19540 - multidrug transporter permease, at 4,083,838 to 4,085,280 _RS19540 BWI76_RS19545: BWI76_RS19545 - type 11 methyltransferase, at 4,085,400 to 4,086,134 _RS19545 BWI76_RS19550: BWI76_RS19550 - phospho-cellobiase, at 4,086,231 to 4,087,625 _RS19550 Position (kb) 4083 4084 4085 4086Strain fitness (log2 ratio) -1 0 1at 4082.925 kb on - strand, within BWI76_RS19530at 4083.031 kb on + strandat 4083.039 kb on - strandat 4083.120 kb on + strandat 4083.254 kb on - strand, within BWI76_RS19535at 4083.273 kb on - strand, within BWI76_RS19535at 4083.432 kb on - strand, within BWI76_RS19535at 4083.767 kb on - strandat 4083.860 kb on + strandat 4084.216 kb on + strand, within BWI76_RS19540at 4084.613 kb on + strand, within BWI76_RS19540at 4084.636 kb on - strand, within BWI76_RS19540at 4084.755 kb on - strand, within BWI76_RS19540at 4085.061 kb on - strand, within BWI76_RS19540

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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4,082,925 - BWI76_RS19530 0.86 +0.9
4,083,031 + -0.1
4,083,039 - +1.2
4,083,120 + -0.0
4,083,254 - BWI76_RS19535 0.16 +0.9
4,083,273 - BWI76_RS19535 0.19 -0.1
4,083,432 - BWI76_RS19535 0.47 +1.2
4,083,767 - +0.1
4,083,860 + +0.3
4,084,216 + BWI76_RS19540 0.26 +0.6
4,084,613 + BWI76_RS19540 0.54 +0.4
4,084,636 - BWI76_RS19540 0.55 +1.1
4,084,755 - BWI76_RS19540 0.64 +0.1
4,085,061 - BWI76_RS19540 0.85 +1.0

Or see this region's nucleotide sequence