Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18295 and BWI76_RS18300 are separated by 30 nucleotides BWI76_RS18300 and BWI76_RS18305 are separated by 66 nucleotides BWI76_RS18305 and BWI76_RS18310 are separated by 190 nucleotides BWI76_RS18310 and BWI76_RS18315 are separated by 13 nucleotides BWI76_RS18315 and BWI76_RS18320 are separated by 38 nucleotides BWI76_RS18320 and BWI76_RS18325 are separated by 151 nucleotides
BWI76_RS18295: BWI76_RS18295 - tyrosine transporter TyrP, at 3,801,476 to 3,802,687
_RS18295
BWI76_RS18300: BWI76_RS18300 - hypothetical protein, at 3,802,718 to 3,803,386
_RS18300
BWI76_RS18305: BWI76_RS18305 - dihydrodipicolinate synthase family protein, at 3,803,453 to 3,804,340
_RS18305
BWI76_RS18310: BWI76_RS18310 - tRNA-Leu, at 3,804,531 to 3,804,617
_RS18310
BWI76_RS18315: BWI76_RS18315 - tRNA-Cys, at 3,804,631 to 3,804,704
_RS18315
BWI76_RS18320: BWI76_RS18320 - tRNA-Gly, at 3,804,743 to 3,804,818
_RS18320
BWI76_RS18325: BWI76_RS18325 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 3,804,970 to 3,805,518
_RS18325
Position (kb)
3803
3804
3805 Strain fitness (log2 ratio)
-1
0
1 at 3802.481 kb on + strand, within BWI76_RS18295 at 3802.672 kb on - strand at 3802.788 kb on - strand, within BWI76_RS18300 at 3802.839 kb on + strand, within BWI76_RS18300 at 3803.016 kb on - strand, within BWI76_RS18300 at 3803.249 kb on - strand, within BWI76_RS18300 at 3803.975 kb on - strand, within BWI76_RS18305 at 3804.128 kb on + strand, within BWI76_RS18305
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,802,481 + BWI76_RS18295 0.83 +0.4 3,802,672 - -0.4 3,802,788 - BWI76_RS18300 0.10 +1.6 3,802,839 + BWI76_RS18300 0.18 -0.7 3,803,016 - BWI76_RS18300 0.45 +0.1 3,803,249 - BWI76_RS18300 0.79 -0.8 3,803,975 - BWI76_RS18305 0.59 +1.4 3,804,128 + BWI76_RS18305 0.76 +0.6
Or see this region's nucleotide sequence