Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16910 and BWI76_RS16915 are separated by 9 nucleotides BWI76_RS16915 and BWI76_RS16920 are separated by 17 nucleotides BWI76_RS16920 and BWI76_RS16925 are separated by 1 nucleotides
BWI76_RS16910: BWI76_RS16910 - 2-keto-4-pentenoate hydratase, at 3,506,554 to 3,507,360
_RS16910
BWI76_RS16915: BWI76_RS16915 - alpha/beta hydrolase fold protein, at 3,507,370 to 3,508,236
_RS16915
BWI76_RS16920: BWI76_RS16920 - 3-(2,3-dihydroxyphenyl)propionate dioxygenase, at 3,508,254 to 3,509,198
_RS16920
BWI76_RS16925: BWI76_RS16925 - 3-(3-hydroxyphenyl)propionate hydroxylase, at 3,509,200 to 3,510,867
_RS16925
Position (kb)
3508
3509
3510 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3507.409 kb on + strand at 3507.410 kb on - strand at 3507.823 kb on - strand, within BWI76_RS16915 at 3507.854 kb on + strand, within BWI76_RS16915 at 3508.140 kb on + strand, within BWI76_RS16915 at 3508.418 kb on + strand, within BWI76_RS16920 at 3508.568 kb on + strand, within BWI76_RS16920 at 3509.124 kb on + strand at 3509.955 kb on - strand, within BWI76_RS16925 at 3510.070 kb on - strand, within BWI76_RS16925 at 3510.179 kb on - strand, within BWI76_RS16925 at 3510.192 kb on + strand, within BWI76_RS16925
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,507,409 + +1.3 3,507,410 - +1.0 3,507,823 - BWI76_RS16915 0.52 +0.2 3,507,854 + BWI76_RS16915 0.56 +1.1 3,508,140 + BWI76_RS16915 0.89 +1.1 3,508,418 + BWI76_RS16920 0.17 -0.4 3,508,568 + BWI76_RS16920 0.33 -2.6 3,509,124 + +2.8 3,509,955 - BWI76_RS16925 0.45 +0.4 3,510,070 - BWI76_RS16925 0.52 -1.4 3,510,179 - BWI76_RS16925 0.59 +0.5 3,510,192 + BWI76_RS16925 0.59 +1.1
Or see this region's nucleotide sequence