Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15075

Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS15070 and BWI76_RS15075 are separated by 65 nucleotidesBWI76_RS15075 and BWI76_RS15080 are separated by 2 nucleotides BWI76_RS15070: BWI76_RS15070 - non-ribosomal peptide synthetase, at 3,153,366 to 3,157,736 _RS15070 BWI76_RS15075: BWI76_RS15075 - non-ribosomal peptide synthase, at 3,157,802 to 3,158,032 _RS15075 BWI76_RS15080: BWI76_RS15080 - hypothetical protein, at 3,158,035 to 3,159,555 _RS15080 Position (kb) 3157 3158 3159Strain fitness (log2 ratio) -2 -1 0 1 2at 3156.880 kb on - strand, within BWI76_RS15070at 3156.880 kb on - strand, within BWI76_RS15070at 3156.968 kb on + strand, within BWI76_RS15070at 3157.182 kb on + strand, within BWI76_RS15070at 3157.252 kb on - strand, within BWI76_RS15070at 3157.280 kb on + strand, within BWI76_RS15070at 3157.313 kb on + strandat 3157.313 kb on + strandat 3157.313 kb on + strandat 3157.321 kb on - strandat 3157.321 kb on - strandat 3157.347 kb on + strandat 3157.355 kb on - strandat 3157.387 kb on - strandat 3157.402 kb on - strandat 3157.547 kb on - strandat 3157.767 kb on + strandat 3157.819 kb on + strandat 3157.827 kb on + strandat 3157.827 kb on + strand, within BWI76_RS15075at 3157.827 kb on + strand, within BWI76_RS15075at 3157.827 kb on + strand, within BWI76_RS15075at 3157.827 kb on - strand, within BWI76_RS15075at 3157.835 kb on - strand, within BWI76_RS15075at 3157.835 kb on - strand, within BWI76_RS15075at 3157.835 kb on - strand, within BWI76_RS15075at 3158.009 kb on - strand, within BWI76_RS15075at 3158.012 kb on + strandat 3158.033 kb on + strandat 3158.033 kb on + strandat 3158.093 kb on + strandat 3158.154 kb on - strandat 3158.197 kb on - strand, within BWI76_RS15080at 3158.268 kb on - strand, within BWI76_RS15080at 3158.270 kb on + strand, within BWI76_RS15080at 3158.366 kb on + strand, within BWI76_RS15080at 3158.371 kb on + strand, within BWI76_RS15080at 3158.471 kb on + strand, within BWI76_RS15080at 3158.476 kb on + strand, within BWI76_RS15080at 3158.476 kb on + strand, within BWI76_RS15080at 3158.535 kb on - strandat 3158.567 kb on + strand, within BWI76_RS15080at 3158.575 kb on - strand, within BWI76_RS15080at 3158.590 kb on - strand, within BWI76_RS15080at 3158.599 kb on - strand, within BWI76_RS15080at 3158.600 kb on - strand, within BWI76_RS15080at 3158.638 kb on - strand, within BWI76_RS15080at 3158.676 kb on - strand, within BWI76_RS15080at 3158.754 kb on - strand, within BWI76_RS15080at 3158.762 kb on + strand, within BWI76_RS15080at 3158.816 kb on - strand, within BWI76_RS15080at 3158.901 kb on + strand, within BWI76_RS15080at 3158.975 kb on + strandat 3158.995 kb on + strand, within BWI76_RS15080at 3158.998 kb on - strand, within BWI76_RS15080

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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3,156,880 - BWI76_RS15070 0.80 -0.1
3,156,880 - BWI76_RS15070 0.80 +0.1
3,156,968 + BWI76_RS15070 0.82 -1.6
3,157,182 + BWI76_RS15070 0.87 +1.0
3,157,252 - BWI76_RS15070 0.89 +0.4
3,157,280 + BWI76_RS15070 0.90 +0.2
3,157,313 + -2.4
3,157,313 + +1.2
3,157,313 + -0.0
3,157,321 - -1.2
3,157,321 - +0.2
3,157,347 + +1.0
3,157,355 - +0.8
3,157,387 - +0.4
3,157,402 - +1.3
3,157,547 - +0.7
3,157,767 + +0.9
3,157,819 + -0.6
3,157,827 + -0.6
3,157,827 + BWI76_RS15075 0.11 -0.7
3,157,827 + BWI76_RS15075 0.11 +1.0
3,157,827 + BWI76_RS15075 0.11 +0.5
3,157,827 - BWI76_RS15075 0.11 +0.5
3,157,835 - BWI76_RS15075 0.14 +0.8
3,157,835 - BWI76_RS15075 0.14 +0.7
3,157,835 - BWI76_RS15075 0.14 +0.9
3,158,009 - BWI76_RS15075 0.90 +0.8
3,158,012 + -0.6
3,158,033 + +0.3
3,158,033 + +0.2
3,158,093 + -0.3
3,158,154 - +2.3
3,158,197 - BWI76_RS15080 0.11 +0.1
3,158,268 - BWI76_RS15080 0.15 +0.5
3,158,270 + BWI76_RS15080 0.15 -0.2
3,158,366 + BWI76_RS15080 0.22 +1.0
3,158,371 + BWI76_RS15080 0.22 -0.6
3,158,471 + BWI76_RS15080 0.29 -0.3
3,158,476 + BWI76_RS15080 0.29 +2.5
3,158,476 + BWI76_RS15080 0.29 +0.7
3,158,535 - -0.6
3,158,567 + BWI76_RS15080 0.35 -1.9
3,158,575 - BWI76_RS15080 0.36 +0.7
3,158,590 - BWI76_RS15080 0.36 -0.3
3,158,599 - BWI76_RS15080 0.37 +1.3
3,158,600 - BWI76_RS15080 0.37 +0.6
3,158,638 - BWI76_RS15080 0.40 +1.3
3,158,676 - BWI76_RS15080 0.42 +0.6
3,158,754 - BWI76_RS15080 0.47 +1.1
3,158,762 + BWI76_RS15080 0.48 +0.3
3,158,816 - BWI76_RS15080 0.51 +0.4
3,158,901 + BWI76_RS15080 0.57 -0.2
3,158,975 + +1.0
3,158,995 + BWI76_RS15080 0.63 -0.0
3,158,998 - BWI76_RS15080 0.63 +2.0

Or see this region's nucleotide sequence