Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14295 and BWI76_RS14300 overlap by 72 nucleotides BWI76_RS14300 and BWI76_RS14305 are separated by 87 nucleotides BWI76_RS14305 and BWI76_RS14310 are separated by 4 nucleotides BWI76_RS14310 and BWI76_RS14315 overlap by 1 nucleotides
BWI76_RS14295: BWI76_RS14295 - IS630 family transposase, at 2,988,739 to 2,989,680
_RS14295
BWI76_RS14300: BWI76_RS14300 - hypothetical protein, at 2,989,609 to 2,989,795
_RS14300
BWI76_RS14305: BWI76_RS14305 - metallophosphoesterase, at 2,989,883 to 2,990,668
_RS14305
BWI76_RS14310: BWI76_RS14310 - hypothetical protein, at 2,990,673 to 2,991,554
_RS14310
BWI76_RS14315: BWI76_RS14315 - MazG nucleotide pyrophosphohydrolase, at 2,991,554 to 2,991,892
_RS14315
Position (kb)
2989
2990
2991 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2988.999 kb on - strand, within BWI76_RS14295 at 2989.053 kb on - strand, within BWI76_RS14295 at 2989.125 kb on - strand, within BWI76_RS14295 at 2989.275 kb on + strand, within BWI76_RS14295 at 2989.275 kb on + strand, within BWI76_RS14295 at 2989.275 kb on + strand, within BWI76_RS14295 at 2989.283 kb on - strand, within BWI76_RS14295 at 2989.283 kb on - strand, within BWI76_RS14295 at 2989.286 kb on + strand, within BWI76_RS14295 at 2989.294 kb on - strand, within BWI76_RS14295 at 2989.452 kb on + strand, within BWI76_RS14295 at 2989.453 kb on + strand, within BWI76_RS14295 at 2989.454 kb on + strand, within BWI76_RS14295 at 2989.462 kb on - strand, within BWI76_RS14295 at 2989.502 kb on + strand, within BWI76_RS14295 at 2989.535 kb on - strand, within BWI76_RS14295 at 2989.665 kb on + strand at 2989.670 kb on + strand at 2989.701 kb on + strand, within BWI76_RS14300 at 2989.715 kb on - strand, within BWI76_RS14300 at 2989.728 kb on + strand, within BWI76_RS14300 at 2989.731 kb on - strand, within BWI76_RS14300 at 2989.731 kb on - strand at 2989.801 kb on + strand at 2989.806 kb on + strand at 2990.042 kb on + strand, within BWI76_RS14305 at 2990.047 kb on + strand, within BWI76_RS14305 at 2990.321 kb on + strand, within BWI76_RS14305 at 2990.390 kb on + strand, within BWI76_RS14305 at 2990.405 kb on + strand, within BWI76_RS14305 at 2990.471 kb on + strand, within BWI76_RS14305 at 2990.471 kb on + strand, within BWI76_RS14305 at 2990.513 kb on + strand, within BWI76_RS14305 at 2990.513 kb on + strand, within BWI76_RS14305 at 2990.838 kb on + strand, within BWI76_RS14310 at 2990.838 kb on + strand, within BWI76_RS14310 at 2990.840 kb on + strand, within BWI76_RS14310 at 2990.840 kb on + strand at 2990.848 kb on + strand, within BWI76_RS14310 at 2990.850 kb on + strand, within BWI76_RS14310 at 2990.985 kb on + strand, within BWI76_RS14310 at 2991.214 kb on + strand, within BWI76_RS14310 at 2991.275 kb on + strand, within BWI76_RS14310 at 2991.275 kb on + strand, within BWI76_RS14310 at 2991.275 kb on + strand, within BWI76_RS14310 at 2991.294 kb on + strand, within BWI76_RS14310 at 2991.307 kb on + strand at 2991.320 kb on + strand, within BWI76_RS14310 at 2991.326 kb on + strand, within BWI76_RS14310 at 2991.327 kb on + strand, within BWI76_RS14310 at 2991.344 kb on + strand, within BWI76_RS14310 at 2991.350 kb on + strand, within BWI76_RS14310 at 2991.374 kb on + strand, within BWI76_RS14310 at 2991.374 kb on + strand, within BWI76_RS14310 at 2991.452 kb on + strand, within BWI76_RS14310 at 2991.491 kb on + strand at 2991.491 kb on + strand at 2991.491 kb on + strand at 2991.491 kb on + strand at 2991.491 kb on + strand at 2991.491 kb on + strand at 2991.491 kb on + strand at 2991.498 kb on + strand at 2991.498 kb on + strand at 2991.498 kb on + strand at 2991.498 kb on + strand at 2991.498 kb on + strand at 2991.498 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,988,999 - BWI76_RS14295 0.28 -0.2 2,989,053 - BWI76_RS14295 0.33 +0.5 2,989,125 - BWI76_RS14295 0.41 -0.2 2,989,275 + BWI76_RS14295 0.57 -0.2 2,989,275 + BWI76_RS14295 0.57 +0.4 2,989,275 + BWI76_RS14295 0.57 +0.4 2,989,283 - BWI76_RS14295 0.58 +0.3 2,989,283 - BWI76_RS14295 0.58 +0.8 2,989,286 + BWI76_RS14295 0.58 -0.3 2,989,294 - BWI76_RS14295 0.59 -0.5 2,989,452 + BWI76_RS14295 0.76 +0.1 2,989,453 + BWI76_RS14295 0.76 +0.1 2,989,454 + BWI76_RS14295 0.76 +0.3 2,989,462 - BWI76_RS14295 0.77 -3.3 2,989,502 + BWI76_RS14295 0.81 -0.3 2,989,535 - BWI76_RS14295 0.85 -0.6 2,989,665 + +0.2 2,989,670 + +1.4 2,989,701 + BWI76_RS14300 0.49 -1.1 2,989,715 - BWI76_RS14300 0.57 -0.9 2,989,728 + BWI76_RS14300 0.64 -1.7 2,989,731 - BWI76_RS14300 0.65 -2.5 2,989,731 - -3.4 2,989,801 + -0.1 2,989,806 + -0.4 2,990,042 + BWI76_RS14305 0.20 +0.5 2,990,047 + BWI76_RS14305 0.21 -2.3 2,990,321 + BWI76_RS14305 0.56 -1.1 2,990,390 + BWI76_RS14305 0.65 -1.9 2,990,405 + BWI76_RS14305 0.66 -1.1 2,990,471 + BWI76_RS14305 0.75 -0.6 2,990,471 + BWI76_RS14305 0.75 -2.5 2,990,513 + BWI76_RS14305 0.80 -2.8 2,990,513 + BWI76_RS14305 0.80 -1.3 2,990,838 + BWI76_RS14310 0.19 -1.4 2,990,838 + BWI76_RS14310 0.19 -1.1 2,990,840 + BWI76_RS14310 0.19 +1.9 2,990,840 + +0.9 2,990,848 + BWI76_RS14310 0.20 +1.2 2,990,850 + BWI76_RS14310 0.20 -0.8 2,990,985 + BWI76_RS14310 0.35 -3.5 2,991,214 + BWI76_RS14310 0.61 -0.3 2,991,275 + BWI76_RS14310 0.68 -1.1 2,991,275 + BWI76_RS14310 0.68 -2.3 2,991,275 + BWI76_RS14310 0.68 +0.9 2,991,294 + BWI76_RS14310 0.70 +0.9 2,991,307 + +2.5 2,991,320 + BWI76_RS14310 0.73 -0.1 2,991,326 + BWI76_RS14310 0.74 +1.2 2,991,327 + BWI76_RS14310 0.74 -0.3 2,991,344 + BWI76_RS14310 0.76 -1.1 2,991,350 + BWI76_RS14310 0.77 +0.3 2,991,374 + BWI76_RS14310 0.79 -1.1 2,991,374 + BWI76_RS14310 0.79 -0.6 2,991,452 + BWI76_RS14310 0.88 -0.4 2,991,491 + -2.1 2,991,491 + +0.2 2,991,491 + -0.4 2,991,491 + -0.7 2,991,491 + +0.2 2,991,491 + -0.1 2,991,491 + +0.9 2,991,498 + -0.1 2,991,498 + -0.7 2,991,498 + +1.3 2,991,498 + -0.5 2,991,498 + -1.4 2,991,498 + +1.5
Or see this region's nucleotide sequence