Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14010

Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14000 and BWI76_RS14005 are separated by 2 nucleotidesBWI76_RS14005 and BWI76_RS14010 are separated by 116 nucleotidesBWI76_RS14010 and BWI76_RS14015 are separated by 265 nucleotides BWI76_RS14000: BWI76_RS14000 - glutamine amidotransferase, at 2,925,897 to 2,926,640 _RS14000 BWI76_RS14005: BWI76_RS14005 - TetR family transcriptional regulator, at 2,926,643 to 2,927,191 _RS14005 BWI76_RS14010: BWI76_RS14010 - SDR family oxidoreductase, at 2,927,308 to 2,928,090 _RS14010 BWI76_RS14015: BWI76_RS14015 - L-2,4-diaminobutyrate decarboxylase, at 2,928,356 to 2,929,828 _RS14015 Position (kb) 2927 2928 2929Strain fitness (log2 ratio) -1 0 1at 2926.339 kb on - strand, within BWI76_RS14000at 2926.343 kb on + strand, within BWI76_RS14000at 2926.523 kb on - strand, within BWI76_RS14000at 2926.570 kb on + strandat 2926.816 kb on + strand, within BWI76_RS14005at 2927.693 kb on + strand, within BWI76_RS14010at 2927.773 kb on + strand, within BWI76_RS14010at 2927.810 kb on + strand, within BWI76_RS14010at 2927.957 kb on + strand, within BWI76_RS14010at 2928.097 kb on + strandat 2928.114 kb on + strandat 2929.084 kb on + strand, within BWI76_RS14015

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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2,926,339 - BWI76_RS14000 0.59 +0.6
2,926,343 + BWI76_RS14000 0.60 +0.8
2,926,523 - BWI76_RS14000 0.84 -0.4
2,926,570 + +0.2
2,926,816 + BWI76_RS14005 0.32 -1.1
2,927,693 + BWI76_RS14010 0.49 +0.5
2,927,773 + BWI76_RS14010 0.59 +1.0
2,927,810 + BWI76_RS14010 0.64 -0.0
2,927,957 + BWI76_RS14010 0.83 +1.5
2,928,097 + -0.2
2,928,114 + +0.5
2,929,084 + BWI76_RS14015 0.49 +0.9

Or see this region's nucleotide sequence