Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12820 and BWI76_RS12825 are separated by 131 nucleotides BWI76_RS12825 and BWI76_RS12830 are separated by 4 nucleotides BWI76_RS12830 and BWI76_RS12835 are separated by 23 nucleotides
BWI76_RS12820: BWI76_RS12820 - NAD-dependent phenylacetaldehyde dehydrogenase, at 2,671,257 to 2,672,756
_RS12820
BWI76_RS12825: BWI76_RS12825 - hypothetical protein, at 2,672,888 to 2,673,619
_RS12825
BWI76_RS12830: BWI76_RS12830 - flagellar motor switch protein FliY, at 2,673,624 to 2,674,454
_RS12830
BWI76_RS12835: BWI76_RS12835 - restriction endonuclease subunit S, at 2,674,478 to 2,675,350
_RS12835
Position (kb)
2672
2673
2674 Strain fitness (log2 ratio)
-2
-1
0
1 at 2671.970 kb on - strand, within BWI76_RS12820 at 2672.084 kb on + strand, within BWI76_RS12820 at 2672.296 kb on - strand, within BWI76_RS12820 at 2672.415 kb on - strand, within BWI76_RS12820 at 2672.527 kb on - strand, within BWI76_RS12820 at 2672.640 kb on - strand at 2672.640 kb on - strand at 2672.899 kb on - strand at 2673.302 kb on + strand, within BWI76_RS12825 at 2674.325 kb on + strand, within BWI76_RS12830 at 2674.345 kb on - strand, within BWI76_RS12830 at 2674.382 kb on - strand at 2674.421 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,671,970 - BWI76_RS12820 0.48 +0.3 2,672,084 + BWI76_RS12820 0.55 +0.5 2,672,296 - BWI76_RS12820 0.69 -1.9 2,672,415 - BWI76_RS12820 0.77 +0.6 2,672,527 - BWI76_RS12820 0.85 -0.6 2,672,640 - -1.4 2,672,640 - -1.0 2,672,899 - +0.3 2,673,302 + BWI76_RS12825 0.57 +1.1 2,674,325 + BWI76_RS12830 0.84 +0.6 2,674,345 - BWI76_RS12830 0.87 -0.6 2,674,382 - -0.4 2,674,421 + +0.4
Or see this region's nucleotide sequence