Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12825

Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12820 and BWI76_RS12825 are separated by 131 nucleotidesBWI76_RS12825 and BWI76_RS12830 are separated by 4 nucleotidesBWI76_RS12830 and BWI76_RS12835 are separated by 23 nucleotides BWI76_RS12820: BWI76_RS12820 - NAD-dependent phenylacetaldehyde dehydrogenase, at 2,671,257 to 2,672,756 _RS12820 BWI76_RS12825: BWI76_RS12825 - hypothetical protein, at 2,672,888 to 2,673,619 _RS12825 BWI76_RS12830: BWI76_RS12830 - flagellar motor switch protein FliY, at 2,673,624 to 2,674,454 _RS12830 BWI76_RS12835: BWI76_RS12835 - restriction endonuclease subunit S, at 2,674,478 to 2,675,350 _RS12835 Position (kb) 2672 2673 2674Strain fitness (log2 ratio) -2 -1 0 1at 2671.970 kb on - strand, within BWI76_RS12820at 2672.084 kb on + strand, within BWI76_RS12820at 2672.296 kb on - strand, within BWI76_RS12820at 2672.415 kb on - strand, within BWI76_RS12820at 2672.527 kb on - strand, within BWI76_RS12820at 2672.640 kb on - strandat 2672.640 kb on - strandat 2672.899 kb on - strandat 2673.302 kb on + strand, within BWI76_RS12825at 2674.325 kb on + strand, within BWI76_RS12830at 2674.345 kb on - strand, within BWI76_RS12830at 2674.382 kb on - strandat 2674.421 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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2,671,970 - BWI76_RS12820 0.48 +0.3
2,672,084 + BWI76_RS12820 0.55 +0.5
2,672,296 - BWI76_RS12820 0.69 -1.9
2,672,415 - BWI76_RS12820 0.77 +0.6
2,672,527 - BWI76_RS12820 0.85 -0.6
2,672,640 - -1.4
2,672,640 - -1.0
2,672,899 - +0.3
2,673,302 + BWI76_RS12825 0.57 +1.1
2,674,325 + BWI76_RS12830 0.84 +0.6
2,674,345 - BWI76_RS12830 0.87 -0.6
2,674,382 - -0.4
2,674,421 + +0.4

Or see this region's nucleotide sequence