Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12795

Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12790 and BWI76_RS12795 are separated by 149 nucleotidesBWI76_RS12795 and BWI76_RS12800 are separated by 195 nucleotides BWI76_RS12790: BWI76_RS12790 - lactate dehydrogenase, at 2,665,125 to 2,666,114 _RS12790 BWI76_RS12795: BWI76_RS12795 - DedA family protein, at 2,666,264 to 2,666,761 _RS12795 BWI76_RS12800: BWI76_RS12800 - hypothetical protein, at 2,666,957 to 2,669,596 _RS12800 Position (kb) 2666 2667Strain fitness (log2 ratio) -1 0 1at 2665.775 kb on + strand, within BWI76_RS12790at 2666.276 kb on + strandat 2666.481 kb on - strand, within BWI76_RS12795at 2666.612 kb on - strand, within BWI76_RS12795at 2666.640 kb on - strand, within BWI76_RS12795at 2666.640 kb on - strand, within BWI76_RS12795at 2666.640 kb on - strand, within BWI76_RS12795at 2666.640 kb on - strand, within BWI76_RS12795at 2666.640 kb on - strand, within BWI76_RS12795at 2666.794 kb on - strandat 2666.968 kb on + strandat 2667.125 kb on - strandat 2667.276 kb on - strand, within BWI76_RS12800at 2667.594 kb on - strand, within BWI76_RS12800at 2667.719 kb on + strand, within BWI76_RS12800

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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2,665,775 + BWI76_RS12790 0.66 -0.4
2,666,276 + +0.4
2,666,481 - BWI76_RS12795 0.44 +1.0
2,666,612 - BWI76_RS12795 0.70 +0.7
2,666,640 - BWI76_RS12795 0.76 -0.0
2,666,640 - BWI76_RS12795 0.76 +1.6
2,666,640 - BWI76_RS12795 0.76 +0.8
2,666,640 - BWI76_RS12795 0.76 +0.5
2,666,640 - BWI76_RS12795 0.76 +0.8
2,666,794 - -0.9
2,666,968 + -0.2
2,667,125 - +0.1
2,667,276 - BWI76_RS12800 0.12 -0.3
2,667,594 - BWI76_RS12800 0.24 -0.0
2,667,719 + BWI76_RS12800 0.29 +0.6

Or see this region's nucleotide sequence