Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12380

Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12375 and BWI76_RS12380 are separated by 488 nucleotidesBWI76_RS12380 and BWI76_RS12385 are separated by 1 nucleotides BWI76_RS12375: BWI76_RS12375 - DNA-invertase hin, at 2,585,407 to 2,585,958 _RS12375 BWI76_RS12380: BWI76_RS12380 - DNA polymerase V subunit UmuD, at 2,586,447 to 2,586,866 _RS12380 BWI76_RS12385: BWI76_RS12385 - DNA polymerase V subunit UmuC, at 2,586,868 to 2,588,133 _RS12385 Position (kb) 2586 2587Strain fitness (log2 ratio) -3 -2 -1 0 1at 2585.536 kb on + strand, within BWI76_RS12375at 2585.614 kb on - strand, within BWI76_RS12375at 2585.644 kb on - strand, within BWI76_RS12375at 2585.800 kb on + strand, within BWI76_RS12375at 2586.058 kb on - strandat 2586.071 kb on + strandat 2586.188 kb on - strandat 2586.460 kb on - strandat 2586.478 kb on + strandat 2586.505 kb on + strand, within BWI76_RS12380at 2586.547 kb on - strand, within BWI76_RS12380at 2586.570 kb on - strand, within BWI76_RS12380at 2586.582 kb on + strand, within BWI76_RS12380at 2586.687 kb on + strand, within BWI76_RS12380at 2586.758 kb on + strand, within BWI76_RS12380at 2586.764 kb on + strand, within BWI76_RS12380at 2586.825 kb on - strandat 2586.827 kb on + strandat 2587.122 kb on - strand, within BWI76_RS12385at 2587.243 kb on + strand, within BWI76_RS12385at 2587.287 kb on + strand, within BWI76_RS12385at 2587.321 kb on + strand, within BWI76_RS12385at 2587.367 kb on + strand, within BWI76_RS12385at 2587.471 kb on + strand, within BWI76_RS12385at 2587.548 kb on - strand, within BWI76_RS12385at 2587.560 kb on + strand, within BWI76_RS12385at 2587.568 kb on - strandat 2587.810 kb on + strand, within BWI76_RS12385

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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2,585,536 + BWI76_RS12375 0.23 +0.1
2,585,614 - BWI76_RS12375 0.38 -0.1
2,585,644 - BWI76_RS12375 0.43 +1.1
2,585,800 + BWI76_RS12375 0.71 -1.6
2,586,058 - -0.9
2,586,071 + -2.5
2,586,188 - -1.1
2,586,460 - -1.3
2,586,478 + -0.2
2,586,505 + BWI76_RS12380 0.14 -0.1
2,586,547 - BWI76_RS12380 0.24 -1.2
2,586,570 - BWI76_RS12380 0.29 -2.0
2,586,582 + BWI76_RS12380 0.32 -0.7
2,586,687 + BWI76_RS12380 0.57 -0.3
2,586,758 + BWI76_RS12380 0.74 -0.3
2,586,764 + BWI76_RS12380 0.75 -0.6
2,586,825 - +0.0
2,586,827 + +0.3
2,587,122 - BWI76_RS12385 0.20 -2.1
2,587,243 + BWI76_RS12385 0.30 -0.9
2,587,287 + BWI76_RS12385 0.33 -0.2
2,587,321 + BWI76_RS12385 0.36 -0.7
2,587,367 + BWI76_RS12385 0.39 -2.8
2,587,471 + BWI76_RS12385 0.48 -1.0
2,587,548 - BWI76_RS12385 0.54 -0.7
2,587,560 + BWI76_RS12385 0.55 -0.8
2,587,568 - -1.5
2,587,810 + BWI76_RS12385 0.74 -2.2

Or see this region's nucleotide sequence