Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS11830

Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntBWI76_RS11825 and BWI76_RS11830 are separated by 154 nucleotidesBWI76_RS11830 and BWI76_RS11835 are separated by 77 nucleotidesBWI76_RS11835 and BWI76_RS11840 are separated by 32 nucleotides BWI76_RS11825: BWI76_RS11825 - LysR family transcriptional regulator, at 2,486,393 to 2,487,292 _RS11825 BWI76_RS11830: BWI76_RS11830 - hypothetical protein, at 2,487,447 to 2,488,394 _RS11830 BWI76_RS11835: BWI76_RS11835 - putative regulatory protein MarR, at 2,488,472 to 2,488,927 _RS11835 BWI76_RS11840: BWI76_RS11840 - tryptophan synthase subunit alpha, at 2,488,960 to 2,489,769 _RS11840 Position (kb) 2487 2488 2489Strain fitness (log2 ratio) -2 -1 0 1 2at 2486.545 kb on + strand, within BWI76_RS11825at 2486.553 kb on - strand, within BWI76_RS11825at 2486.633 kb on + strand, within BWI76_RS11825at 2486.977 kb on + strand, within BWI76_RS11825at 2487.534 kb on - strandat 2487.607 kb on - strand, within BWI76_RS11830at 2487.738 kb on - strand, within BWI76_RS11830at 2487.964 kb on + strand, within BWI76_RS11830at 2488.110 kb on + strand, within BWI76_RS11830at 2488.133 kb on + strand, within BWI76_RS11830at 2488.242 kb on + strand, within BWI76_RS11830at 2488.718 kb on + strand, within BWI76_RS11835at 2488.873 kb on - strand, within BWI76_RS11835at 2489.110 kb on + strand, within BWI76_RS11840at 2489.118 kb on - strand, within BWI76_RS11840at 2489.335 kb on - strand, within BWI76_RS11840

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
remove
2,486,545 + BWI76_RS11825 0.17 +0.9
2,486,553 - BWI76_RS11825 0.18 +0.9
2,486,633 + BWI76_RS11825 0.27 +0.1
2,486,977 + BWI76_RS11825 0.65 +0.6
2,487,534 - +0.8
2,487,607 - BWI76_RS11830 0.17 +0.6
2,487,738 - BWI76_RS11830 0.31 -1.0
2,487,964 + BWI76_RS11830 0.55 +0.9
2,488,110 + BWI76_RS11830 0.70 +2.6
2,488,133 + BWI76_RS11830 0.72 +0.4
2,488,242 + BWI76_RS11830 0.84 +0.6
2,488,718 + BWI76_RS11835 0.54 -0.0
2,488,873 - BWI76_RS11835 0.88 -2.0
2,489,110 + BWI76_RS11840 0.19 -2.0
2,489,118 - BWI76_RS11840 0.20 -2.3
2,489,335 - BWI76_RS11840 0.46 -2.1

Or see this region's nucleotide sequence