Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10535

Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntBWI76_RS10525 and BWI76_RS10530 are separated by 143 nucleotidesBWI76_RS10530 and BWI76_RS10535 overlap by 1 nucleotidesBWI76_RS10535 and BWI76_RS10540 are separated by 16 nucleotides BWI76_RS10525: BWI76_RS10525 - cytosine/purines uracil thiamine allantoin permease, at 2,223,822 to 2,225,309 _RS10525 BWI76_RS10530: BWI76_RS10530 - GntR family transcriptional regulator with, at 2,225,453 to 2,226,169 _RS10530 BWI76_RS10535: BWI76_RS10535 - Asp/Glu/hydantoin racemase, at 2,226,169 to 2,226,912 _RS10535 BWI76_RS10540: BWI76_RS10540 - allantoinase, at 2,226,929 to 2,227,861 _RS10540 Position (kb) 2226 2227Strain fitness (log2 ratio) -2 -1 0 1 2at 2225.283 kb on - strandat 2225.358 kb on - strandat 2225.716 kb on - strand, within BWI76_RS10530at 2225.939 kb on + strand, within BWI76_RS10530at 2226.330 kb on - strand, within BWI76_RS10535at 2226.357 kb on + strand, within BWI76_RS10535at 2226.595 kb on - strand, within BWI76_RS10535at 2226.975 kb on + strandat 2227.349 kb on + strand, within BWI76_RS10540at 2227.392 kb on - strand, within BWI76_RS10540at 2227.618 kb on - strand, within BWI76_RS10540at 2227.644 kb on - strand, within BWI76_RS10540at 2227.667 kb on - strand, within BWI76_RS10540at 2227.754 kb on - strand, within BWI76_RS10540at 2227.888 kb on + strandat 2227.909 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
remove
2,225,283 - +0.5
2,225,358 - +0.5
2,225,716 - BWI76_RS10530 0.37 +1.0
2,225,939 + BWI76_RS10530 0.68 +0.8
2,226,330 - BWI76_RS10535 0.22 +0.8
2,226,357 + BWI76_RS10535 0.25 +1.0
2,226,595 - BWI76_RS10535 0.57 +0.4
2,226,975 + -2.1
2,227,349 + BWI76_RS10540 0.45 -0.4
2,227,392 - BWI76_RS10540 0.50 +2.5
2,227,618 - BWI76_RS10540 0.74 +0.2
2,227,644 - BWI76_RS10540 0.77 +1.0
2,227,667 - BWI76_RS10540 0.79 -0.1
2,227,754 - BWI76_RS10540 0.88 -0.0
2,227,888 + +0.5
2,227,909 - +0.2

Or see this region's nucleotide sequence